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Study on the role of selected cytochrome P450 isoforms in cytostatic resistance at apoptosis level
Moriová, Magdalena ; Hofman, Jakub (advisor) ; Novotná, Eva (referee)
Charles University Faculty of Pharmacy in Hradci Králové Departement of Pharmacology & Toxicology Student: Magdalena Moriová Supervisor: RNDr. Jakub Hofman, Ph.D. Title of diploma thesis: Study on the role of selected cytochrome P450 isoforms in cytostatic resistance at apoptosis level Cytostatic resistance is one of the most problematic obstacles in oncological treatment. Beside pharmacodynamic mechanisms, pharmacokinetic factors play an important role in drug resistance as well. Enzymatic transformation of active substance to inactive metabolite in tumor cells probably belongs to these mechanisms, however, evidences concerning the relevance of this phenomenon are predominantly either indirect and/or affected by interference elements. Using comparative experiments with HepG2 cell lines with/without CYP3A4 overexpression, we focused on the evaluation of the role of this clinically important enzyme in the resistance against docetaxel. Methodologically, it was the assessment of apoptosis induction (activation of caspases 3/7, 8 and 9) using commercial luminescent kits. Our results suggest significant participation of CYP3A4 enzyme on the reduction of docetaxel anticancer efficacy after 48 h from treatment, whereas this effect was not recorded in earlier intervals. These findings perfectly correlate...
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Evaluation of Efficiency and Risks of Private Construction Project
Čičmanec, Juraj ; Novotná, Eva (referee) ; Hromádka, Vít (advisor)
The diploma thesis deals with the evaluation of the effectiveness and risks of a private construction project. This work is divided into two parts. The first part is a theoretical part, which defines the basic concepts associated with investment as such, but also directly with investment in real estate and construction investment. In the second part, this work deals with risk assessment, determination of their size and finally risk management. The end of the theoretical part is the elaboration of methodology for the practical part. The beginning of the practical part the work is devoted to the description of the construction area and specification of the building itself. In the next part are set investment costs, operating income and expenses. Operating income consists of the lease itself. This was determined on the basis of similar investments in the area. Operating expenses are divided into fixed and variable where only fixed are included in the evaluation. Subsequently, the work establishes the profit and loss statement and therefrom the cash flows. The second part defines the risks associated with the construction of the project. The switching value is then calculated for critical project risks. The end of the practical part is devoted to risk management. The conclusion summarizes the results of individual parts of the thesis and a written recommendation for the investor whether to realize the project or not.
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Selective isolation of of the genus Lactobacillus bacteria from foods
Novotná, Eva ; Šárka, Havlíková (referee) ; Rittich, Bohuslav (advisor)
Probiotic lactic acid bacteria of genus Lactobacillus play an important role in the digestive tract of human. They are used in food processing and they are the part of food supplements. Lactic acid bacteria of the genus Lactobacillus can be identificated by polymerase chain reaction (PCR). Bacterial DNA was isolated from cell lysates of 4 synbiotic food suplements by magnetic particles P(HEMA-co-GMA). Isolated DNA was amplified by genus-specific and species-specific primers. Magnetic particles with immobilized antibodies against Lactobacillus bacteria were used in the next part of thesis. These particles were used for isolation target cells from products with their identification by genus specific PCR.
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Valuation of Immovable Cultural Monuments
Novotná, Eva ; Tichá, Alena (referee) ; Komosná, Milada (advisor)
Valuation of immovable cultural monuments is the current topic when cultural heritage is being restored. With its individual character based on the nature of cultural monuments, it is not easy to put forward a uniform award procedure for all cultural monuments. This work aims to create a basic overview of concepts and definitions dealing with issues related to cultural monument, to define the procedure of proclaiming the object as a cultural monument and to focus on valuation procedures with an emphasis on the contribution method.
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Reporting of the Selected Firm
Němec, Vlastimil ; Novotná, Eva (referee) ; Hanušová, Helena (advisor)
This work deals with reporting as a tool for the creation of a comprehensive system of data and indicators testifying about the company's activities in an understandable and user-compact format. Further demonstrated by the practical use of examples and forms of reports to highlight the crucial and often repetitive deficiencies and errors in the use of business information. Based on detailed analyzes are evaluated selected reports and recommendations are defined to make the process of reporting more effective. Selected reports are supplemented by a range of new information that will enhance the management process and therefore lead to savings. These savings are discussed in detail in one of the recommendations of the work on the introduction of CRM IS.
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Application of real-time PCR in microbiological analysis of food-stuffs
Novotná, Eva ; Michaela, Kotianová (referee) ; Španová, Alena (advisor)
Probiotic lactic acid bacteria of genus Lactobacillus play an important role in the digestive tract of human, e.g. protect against pathogenic microorganisms. Lactobacilli are often present in various food products. Lactic acid bacteria of the genus Lactobacillus can be detected by polymerace chain reaction (PCR) using specific primers LbLMA1 and R16. The bacterial DNA was isolated from Actimel Natur probiotic product by the phenol extraction method. DNA was amplified using real time PCR. Specific PCR products were detected using fluorescent intercalaction dye SybrGreen. The specific PCR products were verified by melting curve analysis (Tm 85°C) and by agarose gel electrophoresis (PCR products of 250 bp was amplified).
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Strategic development of the territory in the context of EU policy
Pospíšilová, Radka ; Novotná, Eva (referee) ; Vaňková, Lucie (advisor)
The bachelor thesis deals with territorial development in the context of EU policy. The subject of the theoretical part of the thesis is the sphere of investment and their financing, management of municipalities, EU policy and subsidy programs in the period 2014 – 2020. The practical part analysis the investment in the reconstruction of the transport terminal in the town Holešov. It also includes alternatives to investment financing. Finally, an effect of the investment on the development of the territory is summarized.
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The investment cost of construction
Novotná, Eva ; Brázdová, Eva (referee) ; Marková, Leonora (advisor)
The aim of the thesis is to determine the price of construction investment position, so as to achieve the greatest possible effectiveness for the investor. The theoretical part deals with the life cycle phases, creating price structures and contractual documents. The practical part deals with the application of theory to a case study. Specifically, it examines the lucrative sale of buildings over long-term lease.
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