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Iron metabolism of parasitic amoeba Acanthamoeba castellanii
Krejbichová, Lucie ; Mach, Jan (advisor) ; Horáková, Eva (referee)
Acanthamoeba castellanii, an opportunistic parasitic amoeba, rarely causes infection in humans. Yet, the treatment and diagnosis is often complicated. It causes two diseases: granulomatous encephalitis and acanthamoeba keratitis (inflammation of the cornea of the eye). There is a modern way to deal with parasitic protists and other infectious diseases - interference with iron metabolism. Iron is a crucial element for living organisms; it is involved in many metabolic processes. However, it is also toxic for the organisms and therefore, its uptake, storage and homeostasis in the cell is a strictly controlled process. Ferric reductase takes part in iron homeostasis inside the acanthamoeba cell. Fluorescent microscopy has shown that ferric reductase is located in the vacuole membranes and its activity increases when iron availability decreases. This protein probably mediates the reduction of iron ions that are used in other cellular processes, especially for the transport of iron across membranes. Interestingly, the reduced availability of iron doesn't affect the mitochondrial Fe-S cluster pathway as we proved by fluorescent microscopy with an antibody against one of the proteins of this pathway (IscU). Iron deficiency doesn't affect respiration but alternative oxidase activity is reduced. Moreover, deprived...
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Voltammetric Determination of Genotoxic Nitrobiphenyls
Horáková, Eva ; Vyskočil, Vlastimil (advisor) ; Navrátil, Tomáš (referee)
The presented diploma thesis is devoted to study of determination of 4-nitrobiphenyl (4-NBP) in model river water by DC voltammetry (DCV) and differential pulse voltammetry (DPV) at m-AgSAE (diploma thesis relates to bachelor thesis, in it DCV and DPV methods for determination of 4-NBP in deionized water were developed). Limit of determination (LOD) is 2·10-7 mol·l-1 by DCV and 4·10-7 mol·l-1 by DPV. The adsorptive stripping DPV (AdSDPV) technique was tested to archive lower LOD the AdSDPV, optimal conditions were not found. Electrochemical behavior of 2-nitrobiphenyl (2-NBP) at m-AgSAE was studied. Optimal conditions for its determination by both techniques DCV and DPV were found in methanol-0,01 mol·l-1 LiOH (1:9) and in this medium peak current dependence in 1·10-7 mol·l-1 to 1·10-4 mol·l-1 2-NBP (LOD ≈ 2·10-7 mol·l-1 by DCV and 1·10-7 mol·l-1 by DPV) concentration range was measured. Developed methods were successfully tested for determination of 2-NBP in drinking and river water samples. For both mediums obtained LODs were 2·10-7 mol·l-1 by DCV and 1·10-7 mol·l-1 by DPV. AdSDPV technique was unsuccessfully tested to achieve lower LOD. Optimal conditions for simultaneous determination of 4-NBP and 2-NBP by DPV technique at m-AgSAE were found: methanol-0,25 mol·l-1 acetate buffer (pH = 6,0) 3:7....
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Mercury Electrodes as Tools for Voltammetric Determination of Biologically Active Organic Compounds and for Detection of Their Interaction with DNA
Horáková, Eva ; Vyskočil, Vlastimil (advisor) ; Ludvík, Jiří (referee) ; Vytřas, Karel (referee)
The main aim of this work was to use traditional mercury electrodes for the development of voltammetric methods of determination of organic xenobiotics and for the electrochemical study of the interaction between double-stranded deoxyribonucleic acid (DNA) and these compounds. In relation to my previous research work (conducted in the framework of my diploma thesis), firstly, 4-nitrobiphenyl (4-NBP), the suspected carcinogen, was studied. Interaction of DNA with 4-NBP was studied using differential pulse voltammetry (DPV), cyclic voltammetry (CV), and chronocoulometry at a hanging mercury drop electrode (HMDE), and using CV and alternating current voltammetry at a DNA modified HMDE. Using CV, the reduction mechanism was investigated. The interaction of DNA with 4-aminobiphenyl (4-ABP), a metabolite of 4-NBP, and 4-NBP reduction intermediates was studied. It was found that the interaction of DNA with 4-NBP or 4-ABP results in a formation of a DNA aggregate with these analytes. The second studied analyte was methyl violet 2B (MV). For determination of MV in a buffered solution were used: direct current tast polarography and differential pulse polarography at a dropping mercury electrode, and direct current voltammetry, DPV, and differential pulse adsorptive stripping voltammetry (DPAdSV) at HMDE. The...
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Maintaining dignity for patients in coma at ARO, ICU
Horáková, Eva ; Chloubová, Helena (advisor) ; Herbrichová, Andrea (referee)
This thesis deals with the saturation of the needs of patients in coma. The challenge was to find not only theoretical knowledge of respondents about the needs of man, but especially the higher saturation of psychosocial needs. Work deals with cultural, ethnic and religious diversity of patients and their influence on the patient's dignity. Especially in the health care problems and terminal care. It focuses on the most frequent nursing interventions, which cause disruption of intimacy and thus the stage and dying. In the research part, we try to confirm the objectives and hypotheses using quantitative research methods questionnaire. Quantitative results following qualitative research using observation of randomly selected nurses. From the research showed that nurses do not have sufficient theoretical knowledge of the theory of human needs, and therefore very rarely saturate other than physiological needs. Dignity of a sick person is impaired primarily in health care. Communication not only with the patient, but family is poor and annoying the nurses.
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Voltammetric Determination of 4-Nitrobiphenyl at a Mercury Meniscus Modified Silver Solid Amalgam Electrode
Horáková, Eva ; Barek, Jiří (advisor) ; Schwarzová, Karolina (referee)
The presented bachelor thesis is devoted to study of electrochemical behavior of 4-nitrobiphenyl (4-NBP) and to finding of optimum conditions for its' determination using direct current voltammetry (DCV) and differential pulse voltammetry (DPV) at a mercury meniscus modified silver solid amalgam electrode (m-AgSAE). The aim was to achieve the lowest possible limit of determination and to verify the applicability of the newly developed method for determination of 4-NBP in model samples of drinking water. Methanol-acetate buffer pH 4,8 (3:7) was found as an optimum medium for the determination of 4 NBP at a m-AgSAE in deionized water. During DCV it is not necessary to apply electrochemical regeneration of the working electrode, during DPV it is proper to apply following regeneration potentials: Ereg,1 = 0 mV, Ereg,2 = -1300 mV. The concentration dependence of the peak current was found to be linear for both methods for 10-5, 10-6 and 10-7 mol∙l-1 concentration ranges. The limit of determination of 4-NBP both by DCV and by DPV is 2∙10-7 mol∙l-1. The optimum medium for the determination of 4-NBP in drinking water is the mixture of the sample and acetate buffer pH 4,8 in the 9:1 ratio.. During DCV regeneration was not applied, during DPV it was applied with the following regeneration potentials: Ereg,1...
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Development of reading literacy in selected alternative educational programs
Horáková, Eva ; Wildová, Radka (advisor) ; Ronková, Jolana (referee)
This Master's thesis examines options to advance reading literacy in schools with alternative education. Thesis is focused on teachers work and used methods. The aim of the thesis was to determine, how schools using alternative educational programs support the reading literacy. Theoretical part explores reading literacy, characteristics of alternative educational programs and schools -Montessori, Waldorf, Dalton and program Step by Step. It specifies RWCT - reading and writing to critical thinking program and method of advancement reading literacy and reading. The practical part evaluates surveys filled by teachers from alternative schools and teachers using alternative educational programs. Survey is focused on teacher's job, utilization of RWCT method in schools, working with reading literacy and support of reading.
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Mercury Electrodes as Tools for Voltammetric Determination of Biologically Active Organic Compounds and for Detection of Their Interaction with DNA
Horáková, Eva
The main aim of this work was to use traditional mercury electrodes for the development of voltammetric methods of determination of organic xenobiotics and for the electrochemical study of the interaction between double-stranded deoxyribonucleic acid (DNA) and these compounds. In relation to my previous research work (conducted in the framework of my diploma thesis), firstly, 4-nitrobiphenyl (4-NBP), the suspected carcinogen, was studied. Interaction of DNA with 4-NBP was studied using differential pulse voltammetry (DPV), cyclic voltammetry (CV), and chronocoulometry at a hanging mercury drop electrode (HMDE), and using CV and alternating current voltammetry at a DNA modified HMDE. Using CV, the reduction mechanism was investigated. The interaction of DNA with 4-aminobiphenyl (4-ABP), a metabolite of 4-NBP, and 4-NBP reduction intermediates was studied. It was found that the interaction of DNA with 4-NBP or 4-ABP results in a formation of a DNA aggregate with these analytes. The second studied analyte was methyl violet 2B (MV). For determination of MV in a buffered solution were used: direct current tast polarography and differential pulse polarography at a dropping mercury electrode, and direct current voltammetry, DPV, and differential pulse adsorptive stripping voltammetry (DPAdSV) at HMDE. The...
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Legislativní podmínky pro použití upravených kalů na zemědělské půdě
Andrlová, Veronika ; Horáková, Eva
Tento článek prezentuje současné legislativní aspekty týkající se nakládání s kaly z čistíren odpadních vod. Český zákon o odpadech definoval typy subjektů, které mohou určitým způsobem pracovat s kaly. Vyhláška obsahuje technické podmínky pro použití upravených kalů na zemědělské půdě, například že všechny kaly, které budou takto použity musí být nejdříve upraveny zákonem definovaných zařízeních. Jsou zde popsány evidenční povinnosti, které mají provozovatelé zařízení na použití upravených kalů do půdy.
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Monitoring půdních poměrů v biokoridoru Uhřice
Horáková, Eva
The diploma thesis is focused on the importance of biocorridors in the landscape. Introduction describes the general characterics of biocorridors and their establishment. Practical work was done in the selected biocorridor in Uhřice u Kroměříže. Monitoring was carried out from 2014 to 2016. Soil properties were compared on arable soil, and after its conversion into forest soil and permanent grassland. Following soil properties were observed: texture, soil reaction, conductivity, humus content and quality, nutrient content, hydrophysical properties, and penetrometric soil resistance. Obtained results showed differences in all studied properties after conversion into forest soil and permanent grassland. Conversion into forest soil suggested the highest differences.
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Získávání dat pro hodnocení kvality veřejných prostranství
Kilnarová, Pavla ; Horáková, Eva
Urban spaces hold a lot of essential public functions and play an irreplaceable role in the operating of a modern city. Therefore, monitoring their quality in relation to social needs and the way of its utilization are very important. There are some empirical methods suitable for long-term observation based on direct observation of users’ behaviour in a space that require a rather high number of researchers and are time-consuming. The most frequently used empirical methods for studying public life are the monitoring of pedestrian movement, the monitoring of traffic movement, path tracking and the monitoring of people movement intensity based on the local amenities.
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