National Repository of Grey Literature 6 records found  Search took 0.00 seconds. 
Polymeric nanofibrous carriers for local application of hydrophobic drugs - studying of the drug release profiles by HPLC
Hampejsová, Zuzana
Immunosupresive and cytostatic drugs have many serious side effects which are dose dependent. Local application of drugs prolongs and increases the concentration of drug in the target place and, therefore, may reduce their serious side effects. Polymeric fiber carrier could be used as drug delivery system for local application. Polylactide (PLA) micro/nanofibers containing hydrophobic drugs (immunosupresive drug cyclosporine A and cytostatic drug paclitaxel) were prepared to study this potential medicinal application. Poly(ethylene)glycols (PEG) of various molecular weight (6, 20 and 35 kDa) were incorporated to the structure of fibers to improve compatibility of PLA-drug system and influence the release profiles of hydrophobic drugs. For the systematic development of these materials, it is important to describe the context of the preparation of nanofibers, their morphology and drug release profiles. Therefore, HPLC methods with tandem mass spectrometric or UV detection were optimized and validated to determinate the influence of composition of nanofibers on release kinetics of drugs to different medium (phosphate buffer, hydrogels). The nanofibers with added PEG released significantly higher amounts of drugs and prolonged the release time, compared to the fibers containing only drugs. A multiple...
Polymeric nanofibrous carriers for local application of hydrophobic drugs - studying of the drug release profiles by HPLC
Hampejsová, Zuzana
Immunosupresive and cytostatic drugs have many serious side effects which are dose dependent. Local application of drugs prolongs and increases the concentration of drug in the target place and, therefore, may reduce their serious side effects. Polymeric fiber carrier could be used as drug delivery system for local application. Polylactide (PLA) micro/nanofibers containing hydrophobic drugs (immunosupresive drug cyclosporine A and cytostatic drug paclitaxel) were prepared to study this potential medicinal application. Poly(ethylene)glycols (PEG) of various molecular weight (6, 20 and 35 kDa) were incorporated to the structure of fibers to improve compatibility of PLA-drug system and influence the release profiles of hydrophobic drugs. For the systematic development of these materials, it is important to describe the context of the preparation of nanofibers, their morphology and drug release profiles. Therefore, HPLC methods with tandem mass spectrometric or UV detection were optimized and validated to determinate the influence of composition of nanofibers on release kinetics of drugs to different medium (phosphate buffer, hydrogels). The nanofibers with added PEG released significantly higher amounts of drugs and prolonged the release time, compared to the fibers containing only drugs. A multiple...
Polymeric nanofibrous carriers for local application of hydrophobic drugs - studying of the drug release profiles by HPLC
Hampejsová, Zuzana ; Bosáková, Zuzana (advisor) ; Pacáková, Věra (referee) ; Marie Tlusťáková, Marie (referee)
Immunosupresive and cytostatic drugs have many serious side effects which are dose dependent. Local application of drugs prolongs and increases the concentration of drug in the target place and, therefore, may reduce their serious side effects. Polymeric fiber carrier could be used as drug delivery system for local application. Polylactide (PLA) micro/nanofibers containing hydrophobic drugs (immunosupresive drug cyclosporine A and cytostatic drug paclitaxel) were prepared to study this potential medicinal application. Poly(ethylene)glycols (PEG) of various molecular weight (6, 20 and 35 kDa) were incorporated to the structure of fibers to improve compatibility of PLA-drug system and influence the release profiles of hydrophobic drugs. For the systematic development of these materials, it is important to describe the context of the preparation of nanofibers, their morphology and drug release profiles. Therefore, HPLC methods with tandem mass spectrometric or UV detection were optimized and validated to determinate the influence of composition of nanofibers on release kinetics of drugs to different medium (phosphate buffer, hydrogels). The nanofibers with added PEG released significantly higher amounts of drugs and prolonged the release time, compared to the fibers containing only drugs. A multiple...
Functional role of ISLET1 in the neurosensory development of the inner ear.
Hampejsová, Zuzana ; Pavlínková, Gabriela (advisor) ; Peterka, Miroslav (referee)
Loss of hearing affects more than 10 % of the population, and one newborn in a thousand is born with defects of the inner ear. Transcriptional factors involved in the development of inner ear are important in our understanding of the causes of inner ear defects. ISLET1 is one of these factors. ISLET1 expression is detected in the sensory and neuronal cells of the inner ear. It participates in otocyst formation, and the specification and differentiation of cells of cochlea and vestibular system. The functional role of ISLET1 during inner ear development was investigated. Its role was studied by using Pax2-Isl1 transgenic mice that overexpress Islet1 under the control of the Pax2 promoter. Two transgenic lines were generated, Pax2-Isl1/300 and Pax2- Isl1/52. Two copies of the Pax2-Isl1 transgene were inserted to Pax2-Isl1/300 genome and one copy was inserted to the Pax2-Isl1/52 genome. Defects in sense of hearing were detected in both lines and circling behavior, a defect of balance, was detected in the Pax2-Isl1/300 transgenic mice. We observed high postnatal lethality in heterozygote transgenic mice. Pax2-Isl1/52 homozygote mutation is lethal at embryonic day 10 (E10,5). Pax2-Isl1/300 homozygote letality couldn't be detected because of the inability to breed heterozygote mutated mice of this line....

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