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Microbial production of lipid substances using waste substrates
Árendásová, Veronika ; Szotkowski, Martin (oponent) ; Němcová, Andrea (vedoucí práce)
This bachelor’s thesis examines the issue of lipid production, done through selected Metschnikowia strains gained from waste starch substrates. Produced lipids are extensively used in biotechnological processes. Cultivation utilised waste starch substrates, left over from food production, as they constituted an available and economical material. The study also monitored the growth of individual Metschnikowia strains in laboratory and reduced temperatures, using an optimal medium. The yeast’s production properties were analysed by means of gas chromatography. All selected Metschnikowia strains were able to produce lipid from waste substrates. Lipid production increased when a mixture of glucose and waste substrates was used. The amount of lipids and the share of fatty acids strongly depended on the strain and cultivation conditions. In all examined strains, monounsaturated fatty acids were represented most strongly. The Metschnikowia pulcherima 149 adapted best to waste substrates.
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Use of insect flour for food and feed purposes
Árendásová, Veronika ; Zemanová, Jana (oponent) ; Němcová, Andrea (vedoucí práce)
Insect meal has excellent potential as food or feed. There is a need to provide enough food for the growing population, which is linked to the increasing demand for livestock production. Meat and fish have always been the staple of the human diet as a rich source of proteins and fats for human nutrition. Fish is a good source of animal protein and fat for humans, which forms the basis of the diet of a large number of people who generally live in coastal areas. The increasing demand for fish is associated with a growing interest in high-quality and affordable fish feed. Nowadays, the main ingredient in fish feed is fishmeal, and the price is constantly increasing. The sustainability of the aquaculture industry depends on finding a substitute for fishmeal with the same nutritional value and availability. Recently, there has been a growing interest in animal protein from insects for fish fattening. This thesis focused on analysing insect meal from mealworm larvae (Tenebrio molitor) and its use for food and feed purposes. The theoretical part describes the mealworm, the use of insect meal for human nutrition, and fish fattening. It also describes the requirements of fish for individual nutrients and the characterisation of insects for feeding purposes, focusing on the mealworm used as an alternative feed ingredient in fish. The individual major nutrients, namely protein, lipids, fatty acids, amino acids, fibre, chitin, and selected minerals, were determined in the experimental part. The experimental part was divided into two parts, and the first part was divided into two phases. The first phase was used to determine the nutritional components in two fractions of insect meal from Tenebrio molitor larvae. The first fraction contained the fine fraction, and the second fraction the coarse fraction of insect meal. In the second phase, the content of nutritionally significant components was only determined in the insect meal from dried larvae without fractionation. A fish feed was designed from the analyses results. In the second part, the effect of the addition of insect meal from Tenebrio molitor for food purposes was investigated; specifically, the sensory properties of muffins were monitored. From the results, it can be observed that the nutritional composition of the insect meal suggests the possibility of using the mealworm larvae as an ingredient in the fish diet. The insect meal contains a high proportion of valuable proteins and lipids necessary for fish farming and a low proportion of carbohydrates, which unlike humans, fish do not need in their diet. The sensory analysis results indicate that consumers are not prepared to eat foods with added insects.
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Use of insect flour for food and feed purposes
Árendásová, Veronika ; Zemanová, Jana (oponent) ; Němcová, Andrea (vedoucí práce)
Insect meal has excellent potential as food or feed. There is a need to provide enough food for the growing population, which is linked to the increasing demand for livestock production. Meat and fish have always been the staple of the human diet as a rich source of proteins and fats for human nutrition. Fish is a good source of animal protein and fat for humans, which forms the basis of the diet of a large number of people who generally live in coastal areas. The increasing demand for fish is associated with a growing interest in high-quality and affordable fish feed. Nowadays, the main ingredient in fish feed is fishmeal, and the price is constantly increasing. The sustainability of the aquaculture industry depends on finding a substitute for fishmeal with the same nutritional value and availability. Recently, there has been a growing interest in animal protein from insects for fish fattening. This thesis focused on analysing insect meal from mealworm larvae (Tenebrio molitor) and its use for food and feed purposes. The theoretical part describes the mealworm, the use of insect meal for human nutrition, and fish fattening. It also describes the requirements of fish for individual nutrients and the characterisation of insects for feeding purposes, focusing on the mealworm used as an alternative feed ingredient in fish. The individual major nutrients, namely protein, lipids, fatty acids, amino acids, fibre, chitin, and selected minerals, were determined in the experimental part. The experimental part was divided into two parts, and the first part was divided into two phases. The first phase was used to determine the nutritional components in two fractions of insect meal from Tenebrio molitor larvae. The first fraction contained the fine fraction, and the second fraction the coarse fraction of insect meal. In the second phase, the content of nutritionally significant components was only determined in the insect meal from dried larvae without fractionation. A fish feed was designed from the analyses results. In the second part, the effect of the addition of insect meal from Tenebrio molitor for food purposes was investigated; specifically, the sensory properties of muffins were monitored. From the results, it can be observed that the nutritional composition of the insect meal suggests the possibility of using the mealworm larvae as an ingredient in the fish diet. The insect meal contains a high proportion of valuable proteins and lipids necessary for fish farming and a low proportion of carbohydrates, which unlike humans, fish do not need in their diet. The sensory analysis results indicate that consumers are not prepared to eat foods with added insects.
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Microbial production of lipid substances using waste substrates
Árendásová, Veronika ; Szotkowski, Martin (oponent) ; Němcová, Andrea (vedoucí práce)
This bachelor’s thesis examines the issue of lipid production, done through selected Metschnikowia strains gained from waste starch substrates. Produced lipids are extensively used in biotechnological processes. Cultivation utilised waste starch substrates, left over from food production, as they constituted an available and economical material. The study also monitored the growth of individual Metschnikowia strains in laboratory and reduced temperatures, using an optimal medium. The yeast’s production properties were analysed by means of gas chromatography. All selected Metschnikowia strains were able to produce lipid from waste substrates. Lipid production increased when a mixture of glucose and waste substrates was used. The amount of lipids and the share of fatty acids strongly depended on the strain and cultivation conditions. In all examined strains, monounsaturated fatty acids were represented most strongly. The Metschnikowia pulcherima 149 adapted best to waste substrates.
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