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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Proteom a metabolom parazitů rodu Phytophthora
 Zelená, Pavla
Genus Phytophthora represents a world-wide spread pathogen with more than hundred recognized species and its devastating effect on plants has a serious economic and ecological impact. This diploma thesis entitled „Proteome and metabolome of genus Phytophthora” briefly summarizes knowledge about this pathogen, including its life cycle and interactions with its host. Twelve species representing six Phytophthora clades that were selected for experimental work are then discussed in details. Phytophthora isolates were characterized on proteome and metabolome level employing an LC-MS untargeted proteome profiling and a GC-MS analysis of volatiles. The results were then processed to identify candidate molecules for a targeted identification of Phytophthora and these results were validated in an independent experiment with P. palmivora and Hordeum vulgare. We found that a proteome profiling can be employed as a tool to differentiate individual Phytophthora species and that the marker peptides can be employed for a targeted monitoring of Phytophthora presence in plants.
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Interakce rostlin s pathogeny: význam proteasomu a cytoskeletu
 Zelená, Pavla
Modification of substrate proteins by ubiquitin is one of the major regulatory events in eukaryotic cells, and plants use this modification to react to exogenous and endogenous cues. Mounting evidence indicates that the ubiquitin-proteasome system is also a key component in response to biotic stressors. This thesis entitled "Plant-pathogen interactions: The role of proteasome and cytoskeleton" reviews the role of proteasome in plant signaling and briefly summarizes interactions between cytoskeleton and pathogen and outlines the role of cytokinin in plant-pathogen interactions. In the experimental part, proteasome-mediated signaling is probed using growth response assay, identification of proteasome substrates and confocal microscopy of cytoskeleton structures. The results present evidence that the employed methodology will be useful for plant-pathogen interaction analyses.
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