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The Influence of Elicitation on the Production of Suspension Culture of Trifolium pratense L.
 Zagórová, Marie ; Kašparová, Marie (advisor) ; Dušek, Jaroslav (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate: Marie Zagórová Supervisor: PharmDr. Marie Kašparová, Ph.D. Title of Thesis: The Influence of Elicitation on the Production of Suspension Culture of Trifolium pratense L. The production of secondary metabolites in the Trifolium pratense L. suspension culture (red clover) is low and therefore there was an attempt to increase it by elicitation. Elicitation is a method making use of protective mechanisms of plants to increase the production of secondary metabolites in plants and in vitro cultures. The jasmonic acid and its precursors and derivates are some of the endogeneous signal substances of the plants' defensive responses. In the exogenic application case, they may also function as elicitors. This diploma thesis examined the effect of jasmonic acid and the effect of jasmonic acid in combination with calcium ions and verapamil on the production of flavonoids and isoflavonoids by the Trifolium pratense L. suspension culture. The culture was cultivated in Gamborg medium to which 2 mg.l-1 of 2,4-dichlorophenoxyacetic acid and 2 mg.l-1 of 6-benzylaminopurine were added, at the temperature of 25řC, 16-hr light/8-hr dark period. The flavonoids were determined spectrophotometrically according to the...
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