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Hodnocení kvality ejakulátu beranů vybraných plemen
Wernerová, Karolína
The aim of this thesis was to investigate the issues of ejaculate evaluation and analyse the quality of ejaculates via macroscopic and microscopic methods. From 1st January to 31st March 2015, 69 ejaculates were collected from 7 rams of the dual-purpose Zwartbles breed owned by Ing. Martin Hoška, PhD, 4 rams of the Suffolk breed and 1 ram of the Charollais breed from the School Agricultural Facility Žabčice of Mendel University in Brno. Macroscopic and microscopic analyses were performed immediately after sample collection, including the detection of the ejaculate volume as well as the sperm activity and concentration. For the evaluation of morphological parameters of the ram ejaculates, morphological smears were performed, which were transported to a laboratory of the Department of Animal Breeding, Faculty of Agronomy, Mendel University in Brno. Mainly changes in the sperm head, acrosome, midpiece and tail (torsion, DAG effect) were monitored. Further, the presence of immature and degenerated sperm was also monitored. The results show that the quality of ejaculate is influenced by the age of the rams and particularly by the frequency of the sampling and the size of the testicles whereas the difference between the breeds of distinct purpose was not significant.
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