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The effect of rape seed treatment with oomycete Pythium oligandrum on plant metabolism
Vaverová, Kateřina ; Ryšlavá, Helena (advisor) ; Müller, Karel (referee)
Lot of attention is paid to biological control agents of plant pathogens as it will reduce the amount of pesticides used in agriculture. Pythium oligandrum oomycete is already used commercially in the form of watering and spraying. In this work the properties of other isolates were characterized, and the metabolic changes were studied in plants of oilseed rape (Brassica napus subsp. Oleifera), whose seeds were treated with preparates based on Pythium oligandrum. In the first part of the thesis the properties of compounds secreted by isolates of Pythium oligandrum were tested. The amount of phenolic substances and the activity of endoglycosidase endo-β-1,3-glucanase, cellulase, chitinase as well as proteases were measured to assess the ability of oomycete Pythium oligandrum mycoparasitically protect the plant. Substances, especially oligandrin, which during interaction with the plant are responsible for "priming", have been observed, enabling the plant to respond rapidly to pathogen infection through systemic plant resistance. Differences between individual isolates also manifested themselves in electrophoretic separation in the protein representation. In the second part of the work, the effect of rapeseed seed treatment with isolates of Pythium oligandrum on plant metabolism was monitored. While...
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The effect of temperature and humidity on metabolism of Festuca rubra
Vaverová, Kateřina ; Ryšlavá, Helena (advisor) ; Synková, Helena (referee)
Factors of environment such as availability of water, nutrients, temperature and sunlight affect growth and metabolism of plants. The populations of the Festuca rubra L. from four habitats in Norway, differing in altitude, average temperature and precipitation, were grown in climatic boxes differing in temperature and humidity. The Rubisco, shikimate dehydrogenase activity and amount of the protein were determined in the leaf samples of these plants. The specific activity of Rubisco was the lowest in leaves of Festuca plants grown in drought and elevated temperatures, but statistically significant differences between the various habitats and growth conditions were not found. The specific activity of shikimate dehydrogenase was, on the contrary, under drought conditions and elevated temperatures were the highest. The isoenzyme composition was tested by native electrophoresis. (In Czech) Key words: Rubisco, shikimate dehydrogenase, abiotic stress in plants
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