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Impact of the pre-analytical phase on enzymatic homocysteine determination
 VOSÁTKOVÁ, Eva
This work deals with the stability of homocysteine in blood plasma, it emphasizes the importance of the preanalytical phase of laboratory examination, from the preparation of the patient for blood collection, to the insertion of samples into a fully automatic analyzer. The theoretical part describes homocysteine as such, the diagnostic significance of its examination and describes the laboratory environment. The experimental part monitors the concentration of homocysteine in samples that were processed after a different time interval from blood collection. The measured values of plasma homocysteine concentrations showed that with increasing time lag between sampling and processing itself, the stability of the analyte to be determined is impaired. The stability of plasma homocysteine was also verified in samples processed immediately after blood collection and left at room temperature.
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1 Vosátková, Eliška
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