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Detection of the HLA-B27 allele using a commercial kit
 VOLKOVÁ, Simona
The main topic of the presented work is the detection of the HLA-B27 allele using a commercially available kit from the company BAG HEALTH CARE. The HLA-B27 molecule has been linked to some diseases. The best known is the association with ankylosing spondylitis or Bechterew's disease. Ankylosing spondylitis is a lifelong disease, manifested by an inflammatory reaction mainly at the site of the vertebrae of the spine and large joints such as the hips, shoulders and knees. Thanks to the association, the detection of the HLA-B27 allele can be used as a molecular marker for early diagnosis, mitigation and improvement of the course of treatment of ankylosing spondylitis. The theoretical part of the thesis specifies ankylosing spondylitis and the main histocompatibility complex with emphasis on the key molecule HLA-B27. The practical part of the bachelor thesis is focused on sample collection, DNA isolation and DNA amplification. This is followed by the laboratory detection of the HLA-B27 allele itself using a commercial kit using gel electrophoresis to visualize DNA fragments. Thus, 20 anonymized DNA samples from volunteers were examined. Of the 20 samples examined, positivity for the HLA-B27 allele was demonstrated in 6 samples of volunteers who had different clinical manifestations of Bechterew's disease.
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1 VOLKOVÁ, Šárka
1 Volkova, Sofia
1 Volková, Sandra
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