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Comparison of detection of biofilm formation in clinical strains of Staphylococcus aureus from wounds using culture and genetic methods.
VÁLKOVÁ, Tereza
The main topic of this bachelor thesis is the bacterium Staphylococcus aureus, its ability to form a biofilm and the diagnostics of this formed biofilm. The theoretical part of the thesis first focuses on the bacterium itself, its morphology, antigenic structure and virulence factors. Furthermore, its pathogenesis and pathogenicity will be described, including the issue of the methicillin-resistant Staphylococcus aureus. The next part focuses on the biofilm. The composition, structure and formation of biofilm will be described, as well as its role in pathogenicity, treatment options and various methods of detection. The practical part describes methods of biofilm detection. The first method is crystal violet staining according to Christensen. This method came out positive for all 94 tested strains of Staphylococcus aureus. Subsequently, these strains were divided into categories according to the strength of biofilm production. The second method was Real-time PCR and the detection of selected genes responsible for biofilm formation: clfA, fnbA and fnbB. 2-3 genes were found in 93 samples, and only one sample contained none of the selected genes. Thus, it was shown that all strains are capable of biofilm formation to varying degrees, and no correlation was found between the presence of the examined genes and the level of biofilm production.
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The influence of Musice on Humans
VALKOVÁ, Tereza
The thesis focuses on the description of selected aspects of the influence of music on humans in the context of the psychological point of view and musical functions. It summarizes a person´s relationship to music and provides an overview of positive and negative musical effects. The first part of the thesis describes basic musical terms and some social musical functions. The second part describes the influence the influence of musice on various psychological and physical parts of an individual. The third part points to the music that affects people in day-to-day life.
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Techniques of modeling in kindergarten
VÁLKOVÁ, Tereza
This thesis deals with the modeling techniques in kindergarten. There is an art project created by modeling, which is divided into three groups according to the material used. In each group are two art ideas for working with children. In the theoretical part describes the children's artistic expression, the role of art education in kindergarten, the development of artistic expression, modeling in kindergarten, modeling clay, and especially in modeling methodology management in children. Furthermore ceramics in kindergarten, technical guidelines for working with clay, ceramic materials, glazes and ceramics rules for the novice. The practical part consists of visual modeling project for which they were designed with three ideas art modeling materials. Each theme is described in detail, is given as the procedure needs and motivational activities. These suggestions are verified in practice in three kindergartens. The work is accompanied by photographs.
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