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The study of secondary metabolites in plant tissue cultures II
Urbanová, Nikola ; Kašparová, Marie (advisor) ; Tůmová, Lenka (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Student: Nikola Urbanová Supervisor: PharmDr. Marie Kašparová, Ph.D. Title of diploma thesis: The study of secondary metabolites in plant tissue cultures II The aim of this work was to determine the effect of the potential elicitor chitosan on production of podophyllotoxin in callus and suspension cultures of Juniperus virginiana Glauca variety and suspension cultures of Juniperus virginiana Hetzii variety. Schenk and Hildebrandt medium supplemented with α-naphtylacetic acid (3.0 mg/l), kinetin (0.2 mg/l) and ascorbic acid (15.0 mg/l) was used for the cultivation. Chitosan solutions of various concentrations (0.001; 0.01; 0.1; 1 g/100 ml) were affecting the cultures for 6, 24, 48 and 168 hours. The content of chitosan was determined by HPLC. Higher values of podophyllotoxin content were measured in cultures derived from the Glauca variety. The best chitosan effect on podophyllotoxin production was manifested in callus cultures after 24 hours application of 0.001 g/100ml concentration. A podophyllotoxin content of 0.210 % was determined, which was about 320 % higher in comparison with the control. The maximum content (0.140 %) in suspension culture was induced by 24-hours application of a 0.1 g/100 ml concentration;...
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Knowledge level of first aid procedures among teachers of seleced primary schools in Karlovy Vary
Urbanová, Nikola ; Hanušová, Jaroslava (advisor) ; Váchová, Alena (referee)
This thesis is devoted to Topic: Knowledge level of first aid procedures among teachers of seleced primary schools in Karlovy Vary. In the theoretical part my focus is on the term pre- medical first aid, the principles of providing it, the legislation associated with first aid, mentioned are also the first-aider's safety, the principles of calling professional medical help, there are also the press line phone numbers stated. The thesis contains principles of first aid provided especially to children in selected injury and non-injury conditions. In practical part of this thesis I present quantitative research through questionnaire which was addressed to pedagogues of selected primary and secondary schools in Karlovy Vary. The data obtained were processed into graphs or tables. The main aim of this thesis was to discover the knowledge level of pre-medical first aid in teachers. The questionnaire evaluation suggests that the knowledge level of pre-medical first aid practices in by me selected teachers could be graded as B. It was discovered that the years of pedagogical experience in by me selected teachers might have an impact on the theoretical knowledge of providing pre-medical first aid. It is evident from my research that the best average grade for answering knowledge questions in questionnaire...
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Prevention and prevalence of burnout syndrome among pedagogues of selected primary and secondary schools
Urbanová, Nikola ; Hanušová, Jaroslava (advisor) ; Kučírek, Jiří (referee)
This bachelor thesis takes focus on the burnout syndrom prevention among pedagogic staff in selected primary and high schools. The theoretical part deals with the concept of the burnout syndrom, its causes, phases, risk of its occurrence and last but not least whether the burnout syndrom is a disease or not. The importance of social support and preventive precaution against potential occurrence of the burnout syndrom is also mentioned in this part of the thesis. The practical part is realized through a quantitative research collecting its data in a questionnaire, addressed to pedagogic educators in selected primary and high schools. The receieved data is formed into a graphic illustration. The questionnaire data analysis has shown that the length of pedagogical practice could be in relation to a possible threat of the burnout syndrom. The research results has shown that the most threatened are pedagogues whose practice has lasted for at least 16 years. The survey research has also revealed that almost one half of the respondents experience to be under a threat of the burnout syndrom or perceive themselves to undergo its implications already. Discussion, recommendation and conclusion are another parts of the practical section. Recommendation takes focus on the pedagogues themselves, needs of...
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The study of secondary metabolites in plant tissue cultures II
Urbanová, Nikola ; Kašparová, Marie (advisor) ; Tůmová, Lenka (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Student: Nikola Urbanová Supervisor: PharmDr. Marie Kašparová, Ph.D. Title of diploma thesis: The study of secondary metabolites in plant tissue cultures II The aim of this work was to determine the effect of the potential elicitor chitosan on production of podophyllotoxin in callus and suspension cultures of Juniperus virginiana Glauca variety and suspension cultures of Juniperus virginiana Hetzii variety. Schenk and Hildebrandt medium supplemented with α-naphtylacetic acid (3.0 mg/l), kinetin (0.2 mg/l) and ascorbic acid (15.0 mg/l) was used for the cultivation. Chitosan solutions of various concentrations (0.001; 0.01; 0.1; 1 g/100 ml) were affecting the cultures for 6, 24, 48 and 168 hours. The content of chitosan was determined by HPLC. Higher values of podophyllotoxin content were measured in cultures derived from the Glauca variety. The best chitosan effect on podophyllotoxin production was manifested in callus cultures after 24 hours application of 0.001 g/100ml concentration. A podophyllotoxin content of 0.210 % was determined, which was about 320 % higher in comparison with the control. The maximum content (0.140 %) in suspension culture was induced by 24-hours application of a 0.1 g/100 ml concentration;...
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