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Western blot analysis of ICAM-1 and P-selectin protein expression in aorta of mice with high soluble endoglin levels.
Uhlířová, Barbora ; Rathouská, Jana (advisor) ; Konečná, Klára (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Author: Barbora Uhlířová Supervisor: PharmDr. Jana Rathouská, Ph.D. Title of Diploma Thesis: Western blot analysis of ICAM-1 and P-selectin protein expression in aorta of mice with high soluble endoglin levels Background: Detection and subsequent evaluation of the expression of adhesion molecules ICAM-1 and P-selectin in aorta of two groups of transgenic mice differing by levels of human serum endoglin that were fed a special high fat and cholesterol diet. Methods: We used six-month-old female transgenic mice capable of expressing of human endoglin, which was able to be determined after cleavage in the circulation (Soluble endoglin - sENG) by the use of an ELISA assay. Therefore, we were able to divide the mice into two groups - an experimental group (high levels of sENG) and a control group (very low sENG levels). For 3 months, both groups were given a special high fat and cholesterol diet. Biochemical analysis (total cholesterol levels), and Western blot analysis of molecules ICAM-1 and P-selectin in the homogenate of mouse aortas were carried out. Results: Biochemical analysis of total cholesterol in the blood revealed statistically no significant differences in the measured values...
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Dlouhodobá nezaměstnanost a její dopady na jedince v mikroregionu Boskovicko
Uhlířová, Barbora
The bachelor thesis deals with long-term unemployment and its impact on individuals in the microregion Boskovicko. It is divided into two parts, theoretical and practical. The theoretical part defines the basic concepts related to unemployment. In the practical part of the research conducted to analyze the effects of long-term unemployment in the microregion Boskovicko, then evaluate the results of the investigation and proposed measures to improve the situation of the respondents (in terms of financial, social, physical and psychological health). Conclusion contains recommending proposals arising from qualitative research for labour office in the region.
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Endoparasite infections in sheep and goats in diverse farming systems
UHLÍŘOVÁ, Barbora Isatou
Samples of excrements for parasitological investigation were obtained in two year period 2011 and 2012 on three sheep farms and two goat farms in South Bohemia (a farm conventional, an organic and converting to an organic farming system). A total of 400 samples were examined using four parasitological methods. Thirteen species of gastrointestinal nematodes and one species of Protozoa were recovered. In farms Coccidia Eimeria spp. was the most diagnosed. Results revealed that goats were 1,8 times more often infected with parasites than sheep (x2 = 6,274; d. f. = 1; p = 0, 00576; OR = 1,82). Animals younger 6 months were 3 times more often infected with Eimeria than older ones (x2 = 17,174; d. f. = 1; p = 0,00003; OR = 3,16). Goats were 11 times more often infected with parasites in winter than sheep (x2 = 5,8174; d. f. = 1; p = 0,0050; OR = 10,78). Goats were 2 times more often infected with lungworm infection than sheep (x2 = 8,407; d. f. = 1; p = 0,0019; OR = 1,99). A statistically significant difference in infectious contamination between conventional and an organic system bred animals was not discovered (x2 = 0,0145; d. f. = 1; p = 0,452; OR = 1,05).
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Cryptosporidiosis of small ruminants
UHLÍŘOVÁ, Barbora Isatou
Cryptosporidium (Apicomplexa: Eucoccidiorida: Cryprosporiidae) is a ubiquitous entero-pathogen and has been reported in many geographical regions of the world. Cryptosporidiosis is a zoonotic protozoan disease of worldwide distribution, affecting a wide range of vertebrate hosts. The parasite is recognised as one of the most common causes of diarrhoea in humans and farm animals. There are 23 valid species of Cryptosporidium so far and over 60 genotypes with no species names have been described. Cryptosporidial infection of livestock may have an important economic impact on farmers because of high morbidity and mortality rates among animals. The present study is undertaken to describe problem of cryptosporidiosis of sheep and goats.
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