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Use of synthetic antibodies in immunohistochemistry
Tomečková, Kristýna ; Nejdl,, Lukáš (referee) ; Bezděková,, Jaroslava (advisor)
This diploma thesis deals with the optimization of the preparation of molecularly imprinted (MIP) nanoparticles doped with metal ions, selective for the selected protein. In this work, the model protein – chymotrypsinogen A was chosen as a template. The free radical polymerization method was used for the preparation of molecularly imprinted nanoparticles. Dopamine was used as a functional monomer because it is able to bind metal ions to each other. It also undergoes very rapid oxidative polymerization under alkaline conditions without the need for the addition of polymer reaction initiators. MIP optimization was performed by capillary electrophoresis with UV-Vis detection. The MIP thus prepared can serve as recognition elements in immunohistochemical analyzes that use LA-ICP-MS as a detection method. Their applicability for immunohistochemistry was studied using the dot block method.
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Fluorescence detection in pathogen analysis
Tomečková, Kristýna ; Kristýna, Zemánková (referee) ; Bezděková, Jaroslava (advisor)
This bachelor thesis deals with isolation and detection of bacteria. The used method is called molecular imprinting and is connected with fluorescence microscopy. The theoretical part concentrates on comparison of the developed method with methods that have been used till now. The practical part describes preparation and optimization molecularly imprinted polymers. These polymers were prepared on two different carriers – multititration wellplate and magnetic particles. The bacteria used as imprinted template was Enterococcus faecalis. Staphylococcus aureus was used as its competitor.
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Use of synthetic antibodies in immunohistochemistry
Tomečková, Kristýna ; Nejdl,, Lukáš (referee) ; Bezděková,, Jaroslava (advisor)
This diploma thesis deals with the optimization of the preparation of molecularly imprinted (MIP) nanoparticles doped with metal ions, selective for the selected protein. In this work, the model protein – chymotrypsinogen A was chosen as a template. The free radical polymerization method was used for the preparation of molecularly imprinted nanoparticles. Dopamine was used as a functional monomer because it is able to bind metal ions to each other. It also undergoes very rapid oxidative polymerization under alkaline conditions without the need for the addition of polymer reaction initiators. MIP optimization was performed by capillary electrophoresis with UV-Vis detection. The MIP thus prepared can serve as recognition elements in immunohistochemical analyzes that use LA-ICP-MS as a detection method. Their applicability for immunohistochemistry was studied using the dot block method.
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Fluorescence detection in pathogen analysis
Tomečková, Kristýna ; Kristýna, Zemánková (referee) ; Bezděková, Jaroslava (advisor)
This bachelor thesis deals with isolation and detection of bacteria. The used method is called molecular imprinting and is connected with fluorescence microscopy. The theoretical part concentrates on comparison of the developed method with methods that have been used till now. The practical part describes preparation and optimization molecularly imprinted polymers. These polymers were prepared on two different carriers – multititration wellplate and magnetic particles. The bacteria used as imprinted template was Enterococcus faecalis. Staphylococcus aureus was used as its competitor.
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