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HILIC separation of acyclovir and its degradation product II
Teplá, Lenka ; Pilařová, Pavla (advisor) ; Kastner, Petr (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department: Pharmaceutical chemistry and Drug Control Candidate: Lenka Teplá Tutor: PharmDr. Pavla Pilařová, Ph.D. Title of Thesis: HILIC separation of acyclovir and its degradation product II The aim of this thesis is to test the retention behavior of acyclovir and its degradation product - guanine on zirconia-based column with carbon layer ZirChrom® - CARB under hydrophilic interaction liquid chromatography (HILIC). The thesis also completes the knowledge already obtained from the diploma thesis of Lucie Bouzková (2016), which deals with finding suitable conditions for the separation of analytes. The work examines the influence of the concentration of the phosphoric acid, ammonium fluoride, sodium fluoride, triethylamine and phosphate buffers in the mobile phases on the elution of the analytes. The thesis further looks into the influence of the composition of the mobile phases with different ratios of the organic and aqueous components. The effect of increasing temperature on the retention of acyclovir and guanine was also observed. A higher content of organic component in the mobile phase leads to shortening of retention of acyclovir and guanine. Separation is caused by retention of hydrophilic analytes in the aqueous phase of...
HILIC separation of acyclovir and its degradation product II
Teplá, Lenka ; Pilařová, Pavla (advisor) ; Kastner, Petr (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department: Pharmaceutical chemistry and Drug Control Candidate: Lenka Teplá Tutor: PharmDr. Pavla Pilařová, Ph.D. Title of Thesis: HILIC separation of acyclovir and its degradation product II The aim of this thesis is to test the retention behavior of acyclovir and its degradation product - guanine on zirconia-based column with carbon layer ZirChrom® - CARB under hydrophilic interaction liquid chromatography (HILIC). The thesis also completes the knowledge already obtained from the diploma thesis of Lucie Bouzková (2016), which deals with finding suitable conditions for the separation of analytes. The work examines the influence of the concentration of the phosphoric acid, ammonium fluoride, sodium fluoride, triethylamine and phosphate buffers in the mobile phases on the elution of the analytes. The thesis further looks into the influence of the composition of the mobile phases with different ratios of the organic and aqueous components. The effect of increasing temperature on the retention of acyclovir and guanine was also observed. A higher content of organic component in the mobile phase leads to shortening of retention of acyclovir and guanine. Separation is caused by retention of hydrophilic analytes in the aqueous phase of...
HILIC separation of acyclovir and its degradation product II
Teplá, Lenka ; Pilařová, Pavla (advisor) ; Kastner, Petr (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department: Pharmaceutical chemistry and Drug Control Candidate: Lenka Teplá Tutor: PharmDr. Pavla Pilařová, Ph.D. Title of Thesis: HILIC separation of acyclovir and its degradation product II The aim of this thesis is to test the retention behavior of acyclovir and its degradation product - guanine on zirconia-based column with carbon layer ZirChrom® - CARB under hydrophilic interaction liquid chromatography (HILIC). The thesis also completes the knowledge already obtained from the diploma thesis of Lucie Bouzková (2016), which deals with finding suitable conditions for the separation of analytes. The work examines the influence of the concentration of the phosphoric acid, ammonium fluoride, sodium fluoride, triethylamine and phosphate buffers in the mobile phases on the elution of the analytes. The thesis further looks into the influence of the composition of the mobile phases with different ratios of the organic and aqueous components. The effect of increasing temperature on the retention of acyclovir and guanine was also observed. A higher content of organic component in the mobile phase leads to shortening of retention of acyclovir and guanine. Separation is caused by retention of hydrophilic analytes in the aqueous phase of...
Jakostní parametry masa bažantů
Teplá, Lenka
The bachelor thesis with the theme "Quality parameters of pheasant meat" is engaged in production, composition and quality of meat pheasants. It will also focus on the sensory, physical and microbiological parameters of pheasant meat. The average consumption of game meat is about 1 kg/person/year in the Czech Republic. Pheasant is fattend up 11 -- 24 weeks. A male of pheasant is fed into weighing 1.2 to 1.8 kg and a females is fed into 0.8 to 1.3 kg. The average meat yield is 70%. The average protein content is 24%, fat content is 3.3%, and mineral content is 1.3% in the pheasant meat. Its meat is tasty and aromatic. It is also darker and tougher for us than the more familiar kinds of meats. The pheasant have to leave or hang in cool. Chilled meat is cooled below 4 ° C. The hygiene, the proper processing and avoid microbial contamination during processing is very important.

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2 Teplá, Lucie
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