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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Vliv chloridu sodného na sekundární metabolity u jednobuněčné řasy Chlamydomonas reinhardtii
 Tarbajová, Vladimíra
This thesis studies the effects of various concentrations of sodium chloride on growth and the content of secondary metabolites in the freshwater microalgae Chlamydomonas reinhardtii. The total content of phenolic compounds, flavonoids and total antioxidant capacity was analyzed by spectrophotometry. In the context of growth, also the content of photosynthetic pigments was determined. Further, the amount of selected metabolites was determined by HPLC/MS-MS. Cultivation of microalgae with increased NaCl inhibited cell growth and production of photosynthetic pigments. Conversely, higher levels of NaCl have proven to stimulate the synthesis of complete phenolic compounds and flavonoids. Similarly, the amount of phenolic acids was significantly influenced by the effect of increasing NaCl concentration, while the total antioxidant capacity of the microalgae also increased. These results confirm the involvement of phenolic compounds in the defense mechanism of unicellular algae Ch. reinhardtii against the observed stress factor.
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Interakcia cytokinínov a giberelínov počas predlžovania hypokotylu v odpovedi na rôzne teplotné podmienky
 Tarbajová, Vladimíra
This study observed the involvement of plant hormones, cytokinins during extension of the hypocotyl of Arabidopsis thaliana seedlings ecotype Col-0 in response to ambient temperature. It also observed possible coordination of phytohormones cytokinins with gibberellin pathway, using pentuple della mutant lines background ecotype Ler-0 exposing to elevated temperature. Attention was also paid to regulation of extension growth on the transcriptional level using single and multiple pif mutant lines on a background of the ecotype Col-0. The exogenous application of cytokinin (tZ) demonstrated that elevated levels of cytokinins stimulate the extension growth of the hypocotyl under conditions of optimum temperature (20 ° C). In contrast, the trans-zeatin treated wild type of Arabidopsis thaliana showed inhibition of growth of the hypocotyl under conditions of high ambient temperature (29 ° C). Using a gibberellins´ biosynthesis inhibitor, paclobutrazol, some modulation of hypocotyl elongation thru cytokinins was found in response to the optimal and increased ambient temperature.
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2 Tarbajová, Viera
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