|
|
The assay of peroxidases activities and their comparison in various helminths
Sulanová, Lenka ; Skálová, Lenka (advisor) ; Szotáková, Barbora (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Lenka Sulanová Supervisor: Doc. RNDr. Lenka Skálová, Ph.D. Title of diploma thesis: The assay of peroxidases activities and their comparison in various helminths The aim of diploma thesis was to search for simple methods for determination of peroxidases activities, to choose a suitable method, optimize it and compare it with the method used in the workplace. The 3,3′,5,5′-tetramethylbenzidine (TMB) has been used as a substrate in selected technique. I have optimized this method and compared it with the formely applied method using substrate O-phenylenediamine dihydrochloride (OPD). Method working with TMB is more precise, more comfortable but more expensive than method using OPD. I have applied both of methods for assay of peroxidases activities in the subcellular fractions of the two species of helminths: Dicrocoelicum dendriticum and Haemonchus contortus. I found out that activities of peroxidases are higher at Haemonchus contortus than at Dicrocoelicum dendriticum. Different activities of peroxidases were observed in the subcellular fractions of the Haemonchus contortus strains sensitive and resistant to anthelmintics. The ex vivo contact of Dicrocoelicum dendriticum with...
|
|
| |
|
|
The assay of peroxidases activities and their comparison in various helminths
Sulanová, Lenka ; Skálová, Lenka (advisor) ; Szotáková, Barbora (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Lenka Sulanová Supervisor: Doc. RNDr. Lenka Skálová, Ph.D. Title of diploma thesis: The assay of peroxidases activities and their comparison in various helminths The aim of diploma thesis was to search for simple methods for determination of peroxidases activities, to choose a suitable method, optimize it and compare it with the method used in the workplace. The 3,3′,5,5′-tetramethylbenzidine (TMB) has been used as a substrate in selected technique. I have optimized this method and compared it with the formely applied method using substrate O-phenylenediamine dihydrochloride (OPD). Method working with TMB is more precise, more comfortable but more expensive than method using OPD. I have applied both of methods for assay of peroxidases activities in the subcellular fractions of the two species of helminths: Dicrocoelicum dendriticum and Haemonchus contortus. I found out that activities of peroxidases are higher at Haemonchus contortus than at Dicrocoelicum dendriticum. Different activities of peroxidases were observed in the subcellular fractions of the Haemonchus contortus strains sensitive and resistant to anthelmintics. The ex vivo contact of Dicrocoelicum dendriticum with...
|
guest ::
