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Variabilita mitochondriální DNA ve vztahu k druhové identifikaci živočichů
 Suchá, Františka
Significant tools for identifying animals include molecular genetic methods, particularly DNA barcoding. The aim of this work is to describe methods of DNA barcoding, especially methods of amplification of cytochrom oxidase I (COI) gene. These methods were then used to identify and observe differences between specific examples of species from individual taxonomic groups. For this purpose, samples of insects from Brno and surroundings were collected as well as samples of hair from several selected species of domestic animals. From these samples, DNA was isolated from which the segment of the cytochrom oxidase I (COI) gene was subsequently amplified by PCR. Primers LCO1490/HCO2198 were used for this purpose. This was followed by sequencing and comparison of sequencing results with reference sequences in the databases. Of all 40 samples, 32 samples were amplified and amplification failed only in horsehair samples. The amplified fragments were sequenced successfully and all sequences were assigned to reference sequences in the databases.
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