|
|
Variabilita genů IGFBP2 a MC4R ve vztahu ke kvalitě masa prasat
Steinerová, Michala
The aim of this diploma thesis was association analysis of single nucleotide polymorphism of IGFBP-2 and MC4R genes in a selected group of Czech Large White pigs with indicators of pork quality. The surveyed traits were pH value, color of meat, electrical conductivity, drip loss, contents of intramuscular fat and fatty acids. The polymorphisms were detected by the PCR and RFLP procedures. The allele and genotype frequencies were calculated and results were statistically evaluated using SAS program. In the case of the IGFBP-2 gene was discovered, that the observed set of animals is marked out by the higher percentage of allele B (99,5 %), the relative frequency of the BB genotype was 99 %, AB 1 % and genotype AA has not been found. For this reason, an association analysis could not be performed. The MC4R gene showed a higher frequency of the G allele (74.52%), with the relative frequency of AA genotypes being 2.88%, AG 45.19% and GG 51.92%. On the basis of the association analysis, statistically significant differences were found in the content of the linoleic acid at significance level of P ≤ 0,05 between genotypes AG×GG and to the drip loss where was the difference between the genotypes AA×GG. No statistically significant differences were found for other meat quality indicators, not even value close to significant difference (P ≤ 0,1).
|
|
|
Role polymorfonukleárních leukocytů během zánětlivé odpovědi mléčné žlázy skotu
Steinerová, Michala
The mammary gland is an important production body in dairy cattle breeding. Due to breeding and hybridization programs it reached a position of highly functional exploitation body, which threatens its integrity and homeostasis. The most common health problem of dairy cows is mastitis. As a result, during the evolution, one of the most important defence mechanisms was created, an inflammatory response. It is divided into two stages: stage of initiation and resolution. In both of these stages, polymorphonuclear leukocytes (PMN) play very important role. They migrate from the bloodstream, adhere to the capillary walls, they get through them by diapedesis, and travel to the site of inflammation. There the elimination of pathogens through phagocytosis takes place. Then the damaged tissue is repaired. PMNs undergo apoptosis and they are phagocytosed by macrophages. Therefore, the aim of this work was to study recommended literature and to compile it into the literary research, which would summarize all findings about PMN's role during the inflammatory response of bovine mammary gland.
|
guest ::
RSS feed.