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Role of PML in nucleolar functions
Kučerová, Alena ; Hodný, Zdeněk (advisor) ; Skalníková, Magdalena (referee)
Promyelocytic leukemia protein (PML) is a tumour suppressor which is frequently downregulated in human tumours. PML plays a role in many cellular processes including DNA damage response, senescence and apoptosis and is mainly localized in special structures called PML nuclear bodies (PML NBs). The nucleolus is a key nuclear compartment, where transcription of ribosomal DNA and biogenesis of ribosomes take place. The nucleolus is also called a stress sensor because of its role, for instance, in stabilization of tumour suppressor p53. Localization of PML to the nucleolar periphery appears to be prominent after disturbance of nucleolar functions - for example inhibition of rRNA transcription or processing. Thus the relationship between the nucleolus and PML nuclear bodies may be important for cellular response to stress. However, the role of PML nucleolar associations in nucleolar function including mechanism of formation of these structures remain unclear. Here we characterised PML nucleolar structures and mechanism of their formation. We showed that formation of PML nucleolar structures is not caused by replication stress, is not dependent on any specific phase of cell cycle and is not caused by DNA damage response but is induced by topological stress due to inhibition of toposiomerase function....
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Vliv ročního období na příjem sušiny krmné dávky a produkci mléka u holštýnských dojnic na 1. laktaci
Skalníková, Michaela
In this thesis, main objective was to assess the impact to effect season on quantity remains of feeds with helper PENN STATE separation, and effect on milk production cows at first lactation period from May 2014 to March 2015 in company (ŠZP Žabčice). Monitoring was performed once a month. Laboratory tests determined the dry matter of the remains of feeds and subsequently compared in the dry matter based ratio. From these values were calculated total dry matter intakes from feed cow and the possible impact on the nutritional status of the animals. The results show that, on average, was founded 1908.64 kg feeds in the feed trough, yet remaining 172 kg remains of feeds average for the reporting period. The structure of the mixed ration (TMR) correspond to the recommended values, income dry matter intake cows averaged 22.12 kg / day. Condition score cows ranged on average 3.29 point, and the milk yield in average at 29.37 kg of milk. The exception were the observation in September, when remains of feed has risen strongly and was also observed low dry matter content in TMR, a slight decrease of fitness scores and a decrease in milk production. It was shown that the dry matter intake and the air temperature was negative correlative dependence. In contrast, demonstrated a positive correlates between dry matter intake and milk production.
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Nuclear topography of genes, centromeric heterochromatin and chromosomal domains in human granulocytes
Bártová, Eva ; Kozubek, Stanislav ; Jirsová, Pavla ; Kozubek, Michal ; Lukášová, Emilie ; Skalníková, M. ; Cafourková, Alena ; Koutná, Irena ; Paseková, R.
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