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Studium polymorfizmu DNA mrkve
Sejbalová, Darina
This work is focused on theoretical and practical study of genetic diversity in carrots species. The aim of this work was to analyze the DNA polymorphism in Daucus carota L. by using molecular biology methods based on DNA isolation, PCR and electrophoretic separation. A total of 20 samples were analyzed by using 10 microsatellite markers. It was detected a 50 alleles with an average of five alleles per locus and alleles size between 180 and 380 bp. Further were calculated statistical parameters DI, PI and PIC, which confirmed the high value of polymorphism of used SSR markers. Dendrogram was compiled, identifying the relationships between different varieties of carrots. The results were compared with scientific publications dealing with the same or very similar problems.
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Využití molekulárních markerů ve šlechtění mrkve
Sejbalová, Darina
The aim tis work was study of diversity in carrot (Daucus carota), the analysis of the homogeneity of the parent material, the evaluation of the success of the crossing, and the resolution of the sterile and fertile genotypes of the samples used in crossing. Four hybrid carrot varieties and 12 lines were analyzed, four of which were sterile (CMS), with ten SSR markers and six CMS markers. Based on the calculated statistical values of DI, PI and PIC, the markers were highly variable. A total of 45 alleles were detected, with a mean of 4.5 alleles per locus and 4 zero alleles. The size of the allele ranged between 180 and 380 bp. The success rate of the crossing was evaluated, when the crossing of the Koloseum variety was evaluated as the most successful. Four SSR markers have been recommended as suitable for further study of the diversity and success rate of carrot crosses, namely GSSR-6, GSSR-16, GSSR-91 and GSSR-9. CMS markers successfully identified the sterile and fertile lines at one crossing (variety Koloseum) by Atp8 marker.
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Studium polymorfizmu DNA mrkve
Sejbalová, Darina
This work is focused on theoretical and practical study of genetic diversity in carrots species. The aim of this work was to analyze the DNA polymorphism in Daucus carota L. by using molecular biology methods based on DNA isolation, PCR and electrophoretic separation. A total of 20 samples were analyzed by using 10 microsatellite markers. It was detected a 50 alleles with an average of five alleles per locus and alleles size between 180 and 380 bp. Further were calculated statistical parameters DI, PI and PIC, which confirmed the high value of polymorphism of used SSR markers. Dendrogram was compiled, identifying the relationships between different varieties of carrots. The results were compared with scientific publications dealing with the same or very similar problems.
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