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National Repository of Grey Literature

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Kontinuální analýzy DNA translokace enzymem Typu I EcoR124I Šišáková, Eva ; Seidel, R. ; Szczelkun, M. ; Weiserová, Marie Using combination of bulk solution and single-molecule assays we have investigated the influence of several mutations in the PD-(D/E)-X-K catalytic motif of HsdR on the DNA translocation and ATPase properties of EcoR124I, the restriction-modification enzyme Type I. The gained results have given us the insight into the possible interdomain interactions between helicase and nuclease domains of the HsdR subunit and the effect of their interactions on DNA translocation Detailed record

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