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National Repository of Grey Literature 3 records found  Search took 0.01 seconds. 
Proteins involved in the tetrapyrrole pathway in Synechocystis sp. PCC 6803 and their localization in the proximity of PSII biogenesis
 SKOTNICOVÁ, Petra
The goal of the thesis was to enhance our understanding of the tetrapyrrole pathway in cyanobacteria by a study of selected proteins involved in the pathway. During the project I have revealed functional connection between protoporphyrinogen IX oxidase HemJ and preceding enzyme in the pathway by complementation of protoporphyrinogen IX oxidase deletion mutant by its analog HemG from Escherichia coli. Heme b was identified as a cofactor of HemJ. Another protein deeply influencing tetrapyrrole accumulation, BtpA was found to form a complex with GluTR, the enzyme at the beginning of the tetrapyrrole pathway. Lastly, CurT protein, the component of the structures anticipated to function in PSII assembly and/or repair localized at plasma/thylakoid membrane interface, was co isolated with the enzymes of the tetrapyrrole pathway suggesting that the specific CurT containing membranes could be a place of both photosystem II assembly/repair and chlorophyll delivery.
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Molecular analysis of transgenic rhododendron plants obtained by transformation with 35SGUSint. construct
 SKOTNICOVÁ, Petra
The goal of this thesis was molecular analysis of rhododendron plants obtained after transformation by Agrobacterium tumefaciens and subsequent selection on kanamycin. Presence of incorporated genes gusA and nptII was determined by polymerase chain reaction (PCR) and Southern analysis. GUS activity was determined by fluorimetric and histochemical assay too.
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Verification of transformation of rhododendron leaf segments
 SKOTNICOVÁ, Petra
The selection procedure on kanamycin for rhododendron leaf segments of two species after transformation by Agrobacterium tumefaciens was examined in this thesis. GUS activity and presence of gene gus by polymerase chain reaction (PCR) was determined with plants regenerated on medium with concentration of kanamycin 100mg/l.
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