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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Optimization and Application of Chromatographic and Mass Spectrometric Methods for Determination of Lipidome in Physiological, Nutritional and Biochemical Issues
 SCHNEEDORFEROVÁ, Ivana
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Analysis of Fatty Acids in Diacylglyceroles and Triacylglyceroles of Body Fluids by Gass Chromatography
 SCHNEEDORFEROVÁ, Ivana
Epinephrine is well known a stress hormone of mammals. The adipokinetic hormone (AKH) has the same function in insects. AKH is peptidic hormone; one species of insect possesses one or more types of AKH. Basically AKH is responsible for releasing of diacylglycerols (DGs) from fat body. Our experimental animal is common model organism, locust (Locusta migratoria). Those animal synthesizes three AKH types {--} AKH I, II and III. They differ in amino acid order in peptide, but having very similar influence on the organism. Recently used a routine sulfovaniline method for unsaturated fatty acids (FA) determination estimates only quantitatively the sum of double bounds in fatty acids. Our proposed method, perspective GC FID (Gas Chromatography with Flame Ionization Detector), is very revolutionary being able to determinate particular fatty acid species and also their relative ratio. Aim of this study is to find a proper sample preparation method for GC FID technique and to investigate the influence of particular AKH to the locust organism and to statistically prove the differences in AKH influences. A locust haemolymph was subjected by very usual sample extraction by Folch and a routine SPE (solid phase extraction) method. An easy and rapid derivatization by sodium methanolate was the next sample preparation step followed by GC FID measurement. Acquired data were computed by PCA (principal component analyses). We gained a well-arranged graphical output confirming our thesis that each AKH species has a different influence on FA composition in locust heamolymph; AKH II having the greatest influence.
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