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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Využití různých metod izolace entomopatogenních a mykoparazitických hub z různých podmínek prostředí
 SANDALA, DAVID
Abstract The main objective of this work was to test methods for isolating entomopathogenic fungi from different environmental conditions. In the first experiment, entomopatho-genic fungi B. bassiana, M. brunneum, I. fumosorosea and L. muscarium were inocu-lated in pairs into sterile soil substrate and incubated for 50 days. Compatibility anal-ysis was performed after 14 and 50 days of incubation. The results were compared with those on day 0 when the control suspensions were used to establish tests to assess the number of colony-forming units (CFU). The results showed that entomopathogenic fungi can be easily isolated from soil using the routine method of dodine selective nutrient soil. At the same time, the interaction between species was recorded from photographic documentation. The second experiment focused on the application of the entomopathogenic fungi I. fumosorosea and L muscarium and the mycoparasitic fun-gus T. virens to cucumber plant leaves. The results of this experiment also show that both entomopathogenic fungi and mycoparasitic fungus can be easily isolated even from the phylloplane entomopathogenic fungi using dodine-based selective medium and mycoparasitic fungus T. virens using Trichoderma selective medium (TSM). It was also shown that relative humidity is important for the growth and development of entomopathogenic fungi after preventive application to cucumber plant leaves.
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Vliv teploty na vývoj a virulenci entomopatogenní houby Metarhizium anisopliae
 SANDALA, DAVID
Abstract Entomopathogenic fungus M. brunneum has a high potential in integrated pest management against targeted pests. The commercial bio-product Met52 is based on the strain F52 of entomopathogenic fungus M. brunneum. Temperature is the key factor in the inoculums production of strain F52. The optimal temperature for the cultivation of M. brunneum strain F52 is 25 °C. However, the influence of the temperature of inoculum production plays an important role on the growth and spore production during the subsequent cultivation in different temperature conditions. The optimal temperature for radial grows of strain F52 of M. brunneum from different inoculums is 25 °C and subsequently 30 °C. The cultivation of inoculums at 10 °C appears to be negative because at this temperature the strain grows very slow, and spore production is minimal. The morphology phenotype change of inoculums is influenced by 30 °C of cultivation and also this temperature affects the appearance of inoculum central colonies. The highest production of spores was determined from the colonies established from all inoculums cultivated at 25 °C. The highest mortality and the greatest fungus development was recorded at 25 °C and the most virulent to larvae T. molitor were inoculums produced at 15 °C and 20 °C.
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