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Molecular diagnostic of liver fluke Fascioloides magna.
Siegelová, Veronika ; Kašný, Martin (advisor) ; Sak, Bohumil (referee)
1. ABSTRACT Fascioloides magna (giant liver fluke) is veterinary important endoparasitic helminth parasitizing in a number of vertebrate species (primarily in ruminants), causing them severe health problems, often leading to death. This trematod primarily parasitizing in cervids, but it is also able to infect livestock. The F. magna adults are mostly localized in the liver tissue of the definitive hosts, where they survive in pseudocysts for a long time and produce eggs. The eggs are released from the pseudocysts via the bile ducts into the gut and then leave the host's body together with faeces. Up to now, the diagnostics of fascioloidosis is primarily based on the dissection of the host's liver. The direct and indirect intravital diagnostic methods could represent an appropriate alternative. Using the intravital diagnostic methods, the parasitosis can be revealed directly, microscopically - by discovering the eggs in the host's faeces, but by using this method it is not possible to reliably differentiate the eggs of F. magna from the other eventual trematodes e.g. Fasciola hepatica, Dicrocoelium dendriticum, Paramphistomum cervi. Reliable differentiation of F. magna eggs from the other eventual trematodes can be performed molecularly - by isolation of parasite's DNA followed by PCR. Several methods were...
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