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New interconnections between lipid metabolism and chromatin regulation
Princová, Jarmila ; Převorovský, Martin (advisor) ; Bágeľová Poláková, Silvia (referee) ; Malínský, Jan (referee)
Lipid metabolism has been implicated in changes of chromatin modifications resulting in altered gene expression. Such regulation is important for cellular differentiation or cancer progression, however, the mechanism of how altered metabolic flux leads to targeted changes in chromatin modifications which then regulate gene transcription or heterochromatin maintenance is still poorly understood. We describe that fission yeast cells defective in fatty acid synthesis show increased expression of a subset of stress-response genes. This altered gene expression depends on the SAGA and NuA4 histone acetyltransferases and is associated with increased acetylation of histone H3 at lysine 9 in the corresponding gene promoters. Moreover, diminished fatty acid synthesis results in increased cellular resistance to oxidative stress. Additionally, the lipid metabolism mutants display chromatin alterations in centromeres and subtelomeres, regions of constitutive heterochromatin. We propose that changes in lipid metabolism can regulate histone acetylation and transcription of specific stress-response genes, but also lead to more global changes in heterochromatin. And while we clearly see the consequences of increased stress-response genes which result in promoting redox homeostasis, the implications of altered...
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Ammmonium transport in yeast
Faltýnková, Kateřina ; Palková, Zdena (advisor) ; Princová, Jarmila (referee)
Bachelor thesis - Kateřina Faltýnková - Ammmonium transport in yeast Ammonium and ammonia are an essential nutrient for every yeast cells, not only in metabolism, for example in amino acid synthesis, but also as signalling molecules that serve for communication between colonies or for the regulation of pseudohyphal growth. Transport of ammonia and ammonium ions requires active transport, which is provided by MEP permeases inside the cell likely by exporters ATO proteins out of the cell. In this work there are described families of genes MEP and ATO with main focus on their importance for uptake and export of ammonium ions by yeast and also the regulation of these two gene families in the yeast Saccharomyces cerevisiae, Candida albicans, Cryptococcus neoformans a Yarrowia lipolytica.
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Ammmonium transport in yeast
Faltýnková, Kateřina ; Palková, Zdena (advisor) ; Princová, Jarmila (referee)
Bachelor thesis - Kateřina Faltýnková - Ammmonium transport in yeast Ammonium and ammonia are an essential nutrient for every yeast cells, not only in metabolism, for example in amino acid synthesis, but also as signalling molecules that serve for communication between colonies or for the regulation of pseudohyphal growth. Transport of ammonia and ammonium ions requires active transport, which is provided by MEP permeases inside the cell likely by exporters ATO proteins out of the cell. In this work there are described families of genes MEP and ATO with main focus on their importance for uptake and export of ammonium ions by yeast and also the regulation of these two gene families in the yeast Saccharomyces cerevisiae, Candida albicans, Cryptococcus neoformans a Yarrowia lipolytica.
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