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Zhodnocení léčby parodontálních kostních defektů kompozitním syntetickým materiálem Fortoss? Vital
Sukumar, Sujith ; Dřízhal, Ivo (advisor) ; Poleník, Pavel (referee) ; Korábek, Ladislav (referee)
Background Alloplastic bone graft materials are widely been used these days in combination with barrier membranes to achieve guided tissue regeneration in the treatment of periodontal intrabony defects. This study was designed evaluate the clinical outcome of a composite material, beta tricalcium phosphate in combination with calcium sulphate, in the treatment of periodontal intra-bony defects. The combination of these materials is believed to aid in guided tissue regeneration owing to their properties. Methods Forty seven intrabony defects in 26 periodontitis patients were treated with Fortoss® Vital (Biocomposites, Staffordshire, UK). The patients were followed-up for 2 years. Clinical parameters were evaluated which included changes in probing depth (PD), clinical attachment level/loss (CAL) and gingival recession (GR), presence/absence of plaque and bleeding on probing (BOP) at baseline and at one and two years postoperatively. Results A decrease in probing depths (PD) and a gain in clinical attachment level (CAL) were noticed in at one and two years postoperatively. The mean differences in measurements between the baseline and one year postoperatively are a reduction of 1.97 ± 1.15 mm (p < 0.0001) in case of PD, a gain of 1.68 ± 1.12 mm (p < 0.0001) in CAL and an increase of 0.30 ± 0.71 mm (p...
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Effect of Hyaluronic Acid on Dental PulpStem Cells
Schmidt, Jan ; Suchánek, Jakub (advisor) ; Dostálová, Taťjana (referee) ; Poleník, Pavel (referee)
Hyaluronic acid (HA) and dental pulp stem cells (DPSCs) are attractive research topics and their combined use in tissue engineering is a promising direction in regenerative medicine. DPSCs are a mesenchymal population of stem cells that are obtained from the soft tissues within the dental pulp cavity. Their advantages are easy availability, high proliferative activity, and wide differentiation potential. HA is a natural extracellular biopolymer occurring across human body tissues, including dental pulp. Due to its biocompatibility and biodegradability, HA is a suitable scaffold materal. Degradation of high molecular weight (HMW) HA chains, which are enzymatically cleaved in tissues, results in low molecular weight (LMW) HA fragments. Compared to the HMW HA, LMW HA exhibits markedly different bioactive properties. Thus, it is necessary to assess the effect of HA on tissues and cells separately for its distinct fractions. The results of two in vitro experiments are presented in this work. The first of them was focused on the influence of a wide spectrum of HA (116 kDa, 540 kDa, 1500 kDa) on two DPSC lines. This pilot experiment verified the basic assumption that cells in the environment of selected molecules adhere, survive, proliferate, maintain their typical phenotype and osteogenic and chondrogenic...
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Účinek antimikrobiálních prostředků na mikroorganismy dutiny ústní
Kudiyirickal, Marina George ; Koberová Ivančaková, Romana (advisor) ; Hamal, Petr (referee) ; Poleník, Pavel (referee)
Disease of the pulp and periodontium which constitute the vast proportion of odontogenic infections are mainly caused by the endogenous bacterial microbiota in the oral cavity while non odontogenic infections in the same area vary depending on the nature and site of infection. The rational use of antibiotic is important to prevent development of resistant strains and other side effects of drugs. Aim: To investigate (i) the prevalence of bacterial species in oral samples of patients with bacterial infection reporting at the Dept of Dentistry (1996- 2007), (ii) to assess the age and sex predilection and , (iii) and species specific relationships, and (iv) to determine the susceptible-resistant biotype profile of the bacterial isolates from odontogenic and non odontogenic infections. Materials and methods: Laboratory and clinical data of patient's electronic files at Dept of Microbiology, Faculty of Medicine and Teaching Hospital in Hradec Králové for the years 1996-2007 were evaluated retrospectively. Results: Bacterial orofacial odontogenic or non odontogenic infection was detected in a total of 678 patients with 350 males (51.6%) and 328 females (48.4%). The bacterial isolates included 48 bacterial species with predominance of facultative anaerobes which accounted for 78.5% (n= 1263) and obligate anaerobes...
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Zhodnocení léčby parodontálních kostních defektů kompozitním syntetickým materiálem Fortoss? Vital
Sukumar, Sujith ; Dřízhal, Ivo (advisor) ; Poleník, Pavel (referee) ; Korábek, Ladislav (referee)
Background Alloplastic bone graft materials are widely been used these days in combination with barrier membranes to achieve guided tissue regeneration in the treatment of periodontal intrabony defects. This study was designed evaluate the clinical outcome of a composite material, beta tricalcium phosphate in combination with calcium sulphate, in the treatment of periodontal intra-bony defects. The combination of these materials is believed to aid in guided tissue regeneration owing to their properties. Methods Forty seven intrabony defects in 26 periodontitis patients were treated with Fortoss® Vital (Biocomposites, Staffordshire, UK). The patients were followed-up for 2 years. Clinical parameters were evaluated which included changes in probing depth (PD), clinical attachment level/loss (CAL) and gingival recession (GR), presence/absence of plaque and bleeding on probing (BOP) at baseline and at one and two years postoperatively. Results A decrease in probing depths (PD) and a gain in clinical attachment level (CAL) were noticed in at one and two years postoperatively. The mean differences in measurements between the baseline and one year postoperatively are a reduction of 1.97 ± 1.15 mm (p < 0.0001) in case of PD, a gain of 1.68 ± 1.12 mm (p < 0.0001) in CAL and an increase of 0.30 ± 0.71 mm (p...
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