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Evaluation of drugs mixture toxicity
Podlipská, Kateřina ; Vytlačilová, Jitka (advisor) ; Kašparová, Marie (referee)
Charles University in Prague Pharmaceutical Faculty in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Mgr. Kateřina Podlipská Consultant: RNDr. Jitka Vytlačilová, Ph.D. Title of thesis: Evaluation of drugs mixture toxicity In this thesis were evaluated two substances, ibuprofen, from the group of non-steroidal and anti-inflammatory drugs, and ciprofloxacin, from the group of fluoroquinolone antibiotics. These substances belong to drugs with the highest worldwide consumption and are very often detected in the environment. In this thesis was monitored chronic toxicity of these drugs and their mixtures by using Multigenerational toxicity test with the protozoan Tetrahymena thermophila. For ciprofloxacin was detected the value of 24hEC50 0,12 mg/l (0,11 to 0,14 mg/l), for ibuprofen was 24hEC50 0,18 mg/l (0,15 to 0,22 mg/l), for the mixture of ciprofloxacin and ibuprofen (in ratio 1:1) was 24hEC50 0,08 mg/l (0,07 to 0,10 mg/l), for the mixture of ibuprofen and ciprofloxacin (in ratio 1:2) was 24hEC50 14 mg/l (0,11 to 0,18 mg/l) and for the mixture of ibuprofen and ciprofloxacin (in ratio 2:1) was 24hEC50 0,19 mg/l (0,17 to 0,22 mg/l). Synergistic or additive effect of these two drugs in the mixture was not observed. Keywords: ecotoxicology, ciprofloxacin, chronic toxicity,...
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Evaluation of drugs mixture toxicity
Podlipská, Kateřina ; Vytlačilová, Jitka (advisor) ; Kašparová, Marie (referee)
Charles University in Prague Pharmaceutical Faculty in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Mgr. Kateřina Podlipská Consultant: RNDr. Jitka Vytlačilová, Ph.D. Title of thesis: Evaluation of drugs mixture toxicity In this thesis were evaluated two substances, ibuprofen, from the group of non-steroidal and anti-inflammatory drugs, and ciprofloxacin, from the group of fluoroquinolone antibiotics. These substances belong to drugs with the highest worldwide consumption and are very often detected in the environment. In this thesis was monitored chronic toxicity of these drugs and their mixtures by using Multigenerational toxicity test with the protozoan Tetrahymena thermophila. For ciprofloxacin was detected the value of 24hEC50 0,12 mg/l (0,11 to 0,14 mg/l), for ibuprofen was 24hEC50 0,18 mg/l (0,15 to 0,22 mg/l), for the mixture of ciprofloxacin and ibuprofen (in ratio 1:1) was 24hEC50 0,08 mg/l (0,07 to 0,10 mg/l), for the mixture of ibuprofen and ciprofloxacin (in ratio 1:2) was 24hEC50 14 mg/l (0,11 to 0,18 mg/l) and for the mixture of ibuprofen and ciprofloxacin (in ratio 2:1) was 24hEC50 0,19 mg/l (0,17 to 0,22 mg/l). Synergistic or additive effect of these two drugs in the mixture was not observed. Keywords: ecotoxicology, ciprofloxacin, chronic toxicity,...
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Chronic toxicity of drugs mixture
Podlipská, Kateřina ; Vytlačilová, Jitka (advisor) ; Karlíčková, Jana (referee)
Charles University in Prague Pharmaceutical Faculty in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Podlipská Kateřina Consultant: RNDr. Jitka Vytlačilová, Ph.D. Title of thesis: Chronic toxicity of drugs mixture Ibuprofen, from the group of non-steroidal and anti-inflammatory drugs, and amoxicillin, from the group of broad-spectrum aminopenicillin antibiotics, belong to one of the most often used drugs. In this work was monitored chronic toxicity of these drugs and their mixtures. For the evaluation was selected multigenerational toxicity test with the protozoan Tetrahymena thermophila and algal toxicity test with the alga Desmodesmus subspicatus. It turned out that the more sensitive organism is T. thermophila for which we have determined the value 24hEC50 in the range of 1,53 to 2,57 mg/l compared with the value 72hEC50 for D. subspicatus in the range of 16,77 to 229,3 mg/l. Synergistic action of these two drugs in mixture has not been demonstrated. Keywords: ecotoxicology, amoxicillin, ibuprofen, Tetrahymena thermophila, Desmodesmus subspicatus
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