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Mikrosatelitní panel pro srnčí zvěř a jeho využití
 Podlipná, Eliška
As the name Microsatellite panel for roe deer and its use suggests, my bachelor thesis studies microsatellite markers for roe deer. The goal of this work was to construct microsatellite panel for roe deer and test it, while focusing on genetic variability of roe deer in ŠLP Křtiny and identifying the level of probability of railways and busy roads to be a barrier for the migration of roe deer and their gene flow. A total of 40 roe deer shot in the ŠLP Křtiny area in the 2017-2018 hunting season were sampled. Multiplex PCR was optimized for seven microsatellite markers. Statistical analyses showed presence of one population of roe deer in the ŠLP Křtiny area. Intraspecific differences were observed only on the level of individuals, they were absent on the level of potential groups. Moreover, the study showed that railways and busy roads present no barriers to migration of roe deer or their fluent gene flow. For further studies, I suggest to extend the number of markers for microsatellite panel and the area of roe deer sampling (for example different areas of the Czech Republic).
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