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Effect of dexamethasone on ABCB1 and CYP3A4 expression in human precision-cut intestinal slices
Podhorná, Pavla ; Vokřál, Ivan (advisor) ; Ambrož, Martin (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Pavla Podhorná Supervisor: PharmDr. Ivan Vokřál, Ph.D. Title of diploma thesis: Effect of dexamethasone on ABCB1 and CYP3A4 expression in human precision-cut intestinal slices Dexamethasone is a corticosteroid that has anti-inflammatory, immunosuppressive, and anti-allergic effects. The mechanism of dexamethasone action involves its ability to bind to intracellular glucocorticoid receptors, which are present in many types of cells, including intestinal mucosal cells. Upon binding to these receptors, it translocates to the nucleus of the cells and affects gene expression. This mechanism also affects the expression of genes important for the metabolism and transport of xenobiotics in the intestinal mucosa. The most important such genes include ABCB1, an important intestinal transporter, and CYP3A4, a significant biotransformationenzyme.Their localizationinthe wall of the small intestine can significantlyaffect the absorptionof orallyadministereddrugs.Studyingdrug interactions with this transporter and biotransformation enzyme is important for safe and effective pharmacotherapy. To determine whether dexamethasone affects the expression of these genes in the intestinal barrier,we used the method of...
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