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Role of host-dependent factors in prediction of antiviral treatment response in chronic hepatitis C
Fraňková, Soňa ; Jirsa, Milan (advisor) ; Brůha, Radan (referee) ; Plíšek, Stanislav (referee)
Soňa Fraňková: Role of host-dependent factors in prediction of antiviral treatment response in chronic hepatitis C Abstract Hepatitis C virus infection represents a leading cause of liver disease in western countries. The primary goal of HCV therapy is elimination of the virus, i.e. sustained virological response (SVR) achievement. Genetic factors have long been suspected of playing a crucial role in determining response to IFN-α-based therapies, but pretreatment predictors of response were only poorly defined and did not allow personalization of therapy. The aim of the thesis is to describe the role of host-dependent factors in prediction of antiviral treatment response in chronic hepatitis C in specific groups of patients. First, we focused on the role of the IFNG -764G/C promoter variant in SVR achievement. We did not prove that this variant predicted SVR in Czech HCV-infected individuals. Next, we focused on the role of IL28B and IFNL4 in HCV-infected patients: we confirmed that the IL28B rs12979860 CC genotype slows down the progression of liver fibrosis in chronic HCV infection and that IFNL4 ss469415590 TT|ΔG genotyping does not bring a better prediction of treatment success than IL28B rs12979860 in the Czech population. Third, we assessed prediction of treatment response in HCV positive liver...
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Role of host-dependent factors in prediction of antiviral treatment response in chronic hepatitis C
Fraňková, Soňa ; Jirsa, Milan (advisor) ; Brůha, Radan (referee) ; Plíšek, Stanislav (referee)
Soňa Fraňková: Role of host-dependent factors in prediction of antiviral treatment response in chronic hepatitis C Abstract Hepatitis C virus infection represents a leading cause of liver disease in western countries. The primary goal of HCV therapy is elimination of the virus, i.e. sustained virological response (SVR) achievement. Genetic factors have long been suspected of playing a crucial role in determining response to IFN-α-based therapies, but pretreatment predictors of response were only poorly defined and did not allow personalization of therapy. The aim of the thesis is to describe the role of host-dependent factors in prediction of antiviral treatment response in chronic hepatitis C in specific groups of patients. First, we focused on the role of the IFNG -764G/C promoter variant in SVR achievement. We did not prove that this variant predicted SVR in Czech HCV-infected individuals. Next, we focused on the role of IL28B and IFNL4 in HCV-infected patients: we confirmed that the IL28B rs12979860 CC genotype slows down the progression of liver fibrosis in chronic HCV infection and that IFNL4 ss469415590 TT|ΔG genotyping does not bring a better prediction of treatment success than IL28B rs12979860 in the Czech population. Third, we assessed prediction of treatment response in HCV positive liver...
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The role of molecular genetics methods in diagnostics of neuroinfections
Petroušová, Lenka ; Marešová, Vilma (advisor) ; Göpfertová, Dana (referee) ; Plíšek, Stanislav (referee)
The role of molecular genetics methods in diagnostics of neuroinfections Background: Neuroinfections are serious diseases. They are associated with frequent development of permanent, debilitating consequences or even death. Aim: The aim of this work was to determine possible contribution of molecular genetic methods to diagnosis of neuroinfections and define potentially preventable agents. Methods: The prospective study included 1,463 patients who were hospitalized at the Department of Infectious Diseases in Ostrava in the years 2004-2013 of which 283 patients with purulent meningitis and 1,180 patients with aseptic meningitis. The criterion for the diagnosis was the number of elements in cerebrospinal fluid which had to exceed 5x106 /l. The diagnosis of purulent meningitis was established by cultivation, PCR for the detection of Streptococcus pneumoniae, Haemophilus influenzae, Neisseria meningitidis, and Listeria monocytogenes. The diagnosis of aseptic meningitis was established by serological tests for tick-borne encephalitis, enterovirus, mumps, Mycoplasma pneumoniae, and Lyme disease. PCR was used to investigate enterovirus, influenza virus, VHE and herpes viruses. Result: In case of the 283 patients with purulent meningitis the etiology was established in 73 % cases, PCR method was positive...
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