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Generic drugs and generic substitution-the opinions of pharmacists and their analysis.
Hadravová, Petra ; Vlček, Jiří (advisor) ; Peč, Jaroslav (referee)
Analysis of pharmacist's opinions due to generic drugs and generic substitution Author: Hadravová P. Tutor: Vlček J., Department of Social and Clinical Pharmacy, Faculty of Pharmacy in Hradec Kralove, Charles University in Prague, Czech Republic Background: Generic substitution (GS) came into force by the law in the Czech Republic in January 2008. Followed by a huge discussion about quality and safeness of generic drugs among health professionals. They discussed not only the safety of generic substitution but as well its contribution to the society. However, most of the discussions, were based on unsubstantiated information. Aim of study: This study was focused to gather and analyze the opinions, experiences and knowledge of pharmacists on the issue of generic substitution and generic drugs. Methods: The survey was conducted as a prospective observational cross-sectional study by using questionnaires. Data were collected from November 2008 till April 2009. 7665 members of Czech Chamber of Pharmacists were approached. The collected data were evaluated by descriptive statistics and for evaluation were used selected parametric and nonparametric tests. Results: Six hundred and fifteen pharmacists responded. The return was 8 %. Overall, more women responded (76 %), average age was 38 years. Demographic...
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Study of the Metabolism in in Vitro Cultures of Canabis Sativa L.
Peč, Jaroslav ; Dušek, Jaroslav (advisor) ; Grančai, Daniel (referee) ; Suchý, Václav (referee)
I. Summary The literature review concerning Cannabis sativa L. was prepared with focus on the biologically active compounds. The scope of cell cultures biotechnology and application of modern instrumental analytical methods in the field of metabolomics was discussed. Based on the NMR and MS results, no cannabinoids were detected in the control or elicited cell suspension cultures of Cannabis sativa L. Also other secondary metabolites were not detected by NMR. Other more sensitive analytical methods and their combinations should be used for absolute evaluation of other secondary metabolites, if they are present. It can be concluded, that biosynthesis of the cannabinoids was not induced by pectin or jasmonic acid as elicitors in the suspension, callus or embryogenic tissue cultures of Cannabis sativa L. Main metabolites of certain growt phases were evaluated based on the quantification of produced metabolites and prepared growth phase curves. The impacts of elicitations were interpreted by PCA statistical method, with subsequent metabolite identification or structure elucidation. Maxima of possible 1 H NMR signals were identified and 2D NMR experiments were applied for signals of unknown compounds. The results from NMR measurements combined with PCA analysis brought comprehensive picture of the cultures'...
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