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Microbial lipases and their application
Pavlačková, Jana ; Flodrová, Dana (referee) ; Omelková, Jiřina (advisor)
This diploma thesis is focused on the study of the preparation for fat separators and wastewater pipes that contains the microorganisms with lipolytic activity. Theoretical part of this thesis describes lipases, microorganisms producing this enzymes and usage of lipases. In this part possibilities of identification of microorganisms are presented too. The practical part is concerned with the study of commercial preparation Sany Duo Spezial with proven presence of microorganisms with lipolytic activity. These microorganisms were identified by means of the PCR method. This method identified mictoorganisms like genus Bacillus sp. Next characterization of the preparation was focused on the determination of COD and the investigation of the influence of various conditions of culture medium on the lipases production and their activity. The effect of temperature, ions and pH was studied. Lipolytic activity was determine spectrophotometricaly with usage of p-nitrophenyllaurate whitch dissociates to yellow product p-nitrophenol.
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Microorganisms with lipoplytic activity and their applications
Pavlačková, Jana ; Omelka, Ladislav (referee) ; Omelková, Jiřina (advisor)
Lipases are hydrolytic enzymes that are produced by many types of microorganisms. This thesis describes not only the microorganisms which produce lipases but also different possibilities for the industrial utilization of lipases. Lipases are widely used in reactions where the combination of a lipophilic substrate with a hydrophilic one is necessary – in the synthesis of ascorbic acid fatty esters, sugar esters, lipoaminoacids and in the lipophilization of phenolic derivatives. Lipases are also important in relation to environmental protection. For example, they are used for the purification of waste water. In this thesis, five different preparations containing microorganisms with lipolytic activity were tested for lipolytic activity. There are many ways of determining lipolytic activity. The spectrophotometric determination of lipolytic activity that uses the ability of lipases to divide p-nitrophenyllaurate to p-nitrophenol may serve as an example. After this p-nitophenol is detected spectrophotometricaly.
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Microbial lipases and their application
Pavlačková, Jana ; Flodrová, Dana (referee) ; Omelková, Jiřina (advisor)
This diploma thesis is focused on the study of the preparation for fat separators and wastewater pipes that contains the microorganisms with lipolytic activity. Theoretical part of this thesis describes lipases, microorganisms producing this enzymes and usage of lipases. In this part possibilities of identification of microorganisms are presented too. The practical part is concerned with the study of commercial preparation Sany Duo Spezial with proven presence of microorganisms with lipolytic activity. These microorganisms were identified by means of the PCR method. This method identified mictoorganisms like genus Bacillus sp. Next characterization of the preparation was focused on the determination of COD and the investigation of the influence of various conditions of culture medium on the lipases production and their activity. The effect of temperature, ions and pH was studied. Lipolytic activity was determine spectrophotometricaly with usage of p-nitrophenyllaurate whitch dissociates to yellow product p-nitrophenol.
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Microorganisms with lipoplytic activity and their applications
Pavlačková, Jana ; Omelka, Ladislav (referee) ; Omelková, Jiřina (advisor)
Lipases are hydrolytic enzymes that are produced by many types of microorganisms. This thesis describes not only the microorganisms which produce lipases but also different possibilities for the industrial utilization of lipases. Lipases are widely used in reactions where the combination of a lipophilic substrate with a hydrophilic one is necessary – in the synthesis of ascorbic acid fatty esters, sugar esters, lipoaminoacids and in the lipophilization of phenolic derivatives. Lipases are also important in relation to environmental protection. For example, they are used for the purification of waste water. In this thesis, five different preparations containing microorganisms with lipolytic activity were tested for lipolytic activity. There are many ways of determining lipolytic activity. The spectrophotometric determination of lipolytic activity that uses the ability of lipases to divide p-nitrophenyllaurate to p-nitrophenol may serve as an example. After this p-nitophenol is detected spectrophotometricaly.
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