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Vliv kovů v potravinářství na bakteriální virulenci
 Pavezka, Pavel
The bachelor's thesis deals with the issue of gene transfer of resistance of β-lactam antibiotics by means of horizontal gene transfer, specifically transformation. It deals with the transfer of resistance genes through the food chain, when Escherichia coli can subsequently cause foodborne illness. It also points to the current risks of resistance to β-lactam antibiotics and an alternative method of replacing antibiotics with an iron complex together with an antibiotic. The experimental part is demonstrating the antibacterial effect of the Fe16+AMP complex on the ampicillin-resistant bacterial strain E. coli CCM 4225 and the sensitive strain E. coli CCM 3953. The minimum inhibitory concentration was experimentally determined for these samples. Morphological changes upon exposure of the Fe16+AMP complex to E. coli CCM 3953 were also observed using a cryo-SEM scanning electron microscope. The development of resistance to the model bacterium E. coli TOP10 by transformation was also investigated and verified by polymerase chain reaction PCR and gel electrophoresis. Based on the results, it was proven that the Fe16+AMP complex has an inhibitory effect on the bacterium E. coli CCM 3953 and does not have a significant effect on the resistance spreading via transformation mechanism.
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