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Detection of methicillin - resistant Staphylococcus aureus (MRSA) in Hospital České Budějovice, a. s. and in University Hospital Hradec Králové
Polenová, Lucie ; Paterová, Pavla (advisor) ; Hobzová, Lenka (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Student: Bc. Lucie Polenová Supervisor: MUDr. Pavla Paterová Title of diploma thesis: Detection of methicillin - resistant Staphylococcus aureus (MRSA) in Hospital České Budějovice, a. s. and in University Hospital Hradec Králové The resistance of microbes to antibiotics belongs to worldwide health problems. Infections, which are caused by methicillin-resistant Staphylococcus aureus (MRSA), occur mainly in hospitals. It is because of incorrect and excesive consumption of antibiotics. Insufficient observance of hygienic-epidemiological measures helps to spread resistant strains. Background: The aim of the study was to determine the incidence of MRSA strains in two Czech hospitals - in Hospital České Budějovice, a.s. and in University Hospital Hradec Králové. To compare results with figures in the previous years and sort isolated strains by different characteristics in both hospitals. Methods: Results of the study are based on retrospective data analysis from electronic database. All data from hospitalized patients or outpatients during 1.1.2015 - 31.12.2015 with isolation of MRSA were counted. Processing and cultivation of isolates were made by standard microbiology measures. Strains,...
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Detection of methicillin - resistant Staphylococcus aureus (MRSA) in Hospital České Budějovice, a. s. and in University Hospital Hradec Králové
Polenová, Lucie ; Paterová, Pavla (advisor) ; Hobzová, Lenka (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Student: Bc. Lucie Polenová Supervisor: MUDr. Pavla Paterová Title of diploma thesis: Detection of methicillin - resistant Staphylococcus aureus (MRSA) in Hospital České Budějovice, a. s. and in University Hospital Hradec Králové The resistance of microbes to antibiotics belongs to worldwide health problems. Infections, which are caused by methicillin-resistant Staphylococcus aureus (MRSA), occur mainly in hospitals. It is because of incorrect and excesive consumption of antibiotics. Insufficient observance of hygienic-epidemiological measures helps to spread resistant strains. Background: The aim of the study was to determine the incidence of MRSA strains in two Czech hospitals - in Hospital České Budějovice, a.s. and in University Hospital Hradec Králové. To compare results with figures in the previous years and sort isolated strains by different characteristics in both hospitals. Methods: Results of the study are based on retrospective data analysis from electronic database. All data from hospitalized patients or outpatients during 1.1.2015 - 31.12.2015 with isolation of MRSA were counted. Processing and cultivation of isolates were made by standard microbiology measures. Strains,...
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Detection of extended-spectrum beta-lactamase (ESBL) producers in community and in a hospital
POLENOVÁ, Lucie
The incidence of bacteria resistant to available antibiotics has become an important problem. Statistics show the rising number of resistant bacteria worldwide. Bacteria have ability to be resistant naturally or they develop from point mutation of genes and relay it between species or kinds. The study was made because of significance and relevance of this problem. In theoretical part the study is focused on the functional parts of bacterial cell, which participate in mechanisms of resistance. Beta - lactam antibiotics are described in more detail. The research took place in Hospital České Budějovice, a. s. during 2013. The study aimed to determine the frequency of ESBL positive producers in this hospital and to compare with frequency in community, and to identify units with highest frequency of ESBL producers. Pathogens are isolated from clinical material, being delivered to bacteriology lab in Hospital České Budějovice, a. s. Resistant samples were examined with double disc synergy test (DDST) and quantitative method based on principle of minimal inhibition concentration in combination with chromogenic agar. The prevalence rate of ESBL producers is average to low in Hospital České Budějovice, a. s. During 2013, 12, 347 isolates were identified, ESBL production was identified in 226 isolates. Escherichia coli (183 isolates), Klebsiella pneumoniae (16 isolates), and Enterobacter cloacae (15 isolates) were the most prevalent species. The number of ESBL producers was lower compared to 2012, 2011, and 2010.
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