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Isolace mikroorganismů z mlék a mléčných výrobků schopných tvořit biogenní aminy
Přichystalová, Jitka
The aim of the dissertation thesis, The Isolation of Microorganisms Producing Biogenic Amines from Milks and Dairy Products, was to isolate pure bacterial cultures of the genus Enterococcus and coliform bacteria from milks and cheeses and to test them for ability to produce biogenic amines. Isolated strains were identified and decarboxylase activity was tested on several amino-acids (lysine, histidine, tyrosine, tryptamine, ornithine, phenylalanine and arginine) at four different temperatures. At the end of the experiment, PCR was processed for the presence of tyrosine decarboxylase gene in enterococci strains. Identification showed higher variability of microorganisms isolated from cheeses rather than from milks. Decarboxylation activity test has revealed ability of genus Enterococcus to decarboxylate tyrosin and arginine. Coliform bacteria were found to be possibly able to decarboxylate arginine, tyrosin, ornithine, lysine and histidine. The subsequent PCR reaction confirmed most of the enterococci isolates to be producers of tyramine.
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