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Differentiation in the polyploid complex Libanotis pyrenaica (Apiaceae)
Přívozníková, Hana ; Chrtek, Jindřich (advisor) ; Vít, Petr (referee)
Polyploidization belong to principal evolutionary mechanisms in plants. Although it is rather rare in the family Apiaceae, there are several interesting polyploid complexes here. The present study deals with Libanotis pyrenaica, species three previously reported ploidy levels, namely diploid (2n = 2x = 22), tetraploid (2n = 4 x = 44) and most likely very rare triploid (2n = 3x = 33). The present study aims at geographical pattern of ploidal diversity in central Europe, genetic variation and selected biological features of this species. Flow cytometru, analysis of two chloroplast DNA markers (trnF-trnL and cp018-cp020), morphometrics and in vitro germination experiments were used. The tetraploid cytotype occurs throughout the studied area; in the Czech Republic it was found in the České středohoří Mts., southern Bohemia and Moravia, in Slovakia it especially in the Carpathian mountains in western and central parts of the country and in the Pieniny Mts. in the northeast. Tetraploid cytotype occurs mainly in central-east Europe; in the Czech Republic it is common in the lowlands along the river of Labe and locally in Moravia; in Slovakia it was found along the river of Váh, in the Slovenské rudohorie Mts. and in the Belanské Tatry Mts. A mixed-ploidy population (2x, 4x) was discovered in southern...
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Differentiation in the polyploid complex Libanotis pyrenaica (Apiaceae)
Přívozníková, Hana ; Chrtek, Jindřich (advisor) ; Vít, Petr (referee)
Polyploidization belong to principal evolutionary mechanisms in plants. Although it is rather rare in the family Apiaceae, there are several interesting polyploid complexes here. The present study deals with Libanotis pyrenaica, species three previously reported ploidy levels, namely diploid (2n = 2x = 22), tetraploid (2n = 4 x = 44) and most likely very rare triploid (2n = 3x = 33). The present study aims at geographical pattern of ploidal diversity in central Europe, genetic variation and selected biological features of this species. Flow cytometru, analysis of two chloroplast DNA markers (trnF-trnL and cp018-cp020), morphometrics and in vitro germination experiments were used. The tetraploid cytotype occurs throughout the studied area; in the Czech Republic it was found in the České středohoří Mts., southern Bohemia and Moravia, in Slovakia it especially in the Carpathian mountains in western and central parts of the country and in the Pieniny Mts. in the northeast. Tetraploid cytotype occurs mainly in central-east Europe; in the Czech Republic it is common in the lowlands along the river of Labe and locally in Moravia; in Slovakia it was found along the river of Váh, in the Slovenské rudohorie Mts. and in the Belanské Tatry Mts. A mixed-ploidy population (2x, 4x) was discovered in southern...
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Libanotis pyrenaica - a unique polyploid complex in the family Apiaceae
Přívozníková, Hana ; Chrtek, Jindřich (advisor) ; Vít, Petr (referee)
6 Abstract Libanotis pyrenaica is sometimes neglected representative of Czech flora, a member of Apiaceae family. It grows in xerothermic habitats, on basic soils, particularly on limestones. It can be found on shrubby, stony or grassy slopes, screes, in quarries, pastures, light forests, along the roads and railroads, river banks and in castle ruins. Libanotis pyrenaica is native to the Czech Republic, but is spreading secondarily to many habitats (along the river banks, railroads). Its secondary occurrence has been documented in e.g. Eastern Bohemia. There are two ploidy levels known in the Czech Republic: 2n = 2x = 22 and 2n = 4x = 44. Diploid plants were found in Southern Bohemia (limestones of Sušicko-Horaždovice region, Českokrumlovské Předšumaví region), while the tetraploid plants were detected in Eastern Bohemia and Southern Moravia. In contrast, diploids seem to prevail throughout Europe. However, exact frequency, geographical occurrence and differentiation of both ploidies are unknown up to date. Key words: Libanotis pyrenaica, Seseli, Moon Carrot, diploid, tetraploid, cytotype differentiation, Apiaceae, flow cytometry
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