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Environmental risk of nanoparticles
Píchová, Magdalena ; Szotáková, Barbora (advisor) ; Karlíčková, Jana (referee)
CharlesUniversity in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Magdalena Píchová Supervisor: Doc. Ing. Barbora Szotáková, Ph.D. Consultant: RNDr. Radka Podlipná, Ph.D. Title of diploma thesis: Environmental risk of nanoparticles The goal of this diploma thesis was to evaluate the influence of various heavy metal oxides in the form of nanoparticles on plants. There were four elements examined: copper, iron, manganese and zinc. For the comparison the ordinary form of the oxide, soluble salt (sulphate) and plants growing on the medium were used. The research was conducted using white mustard (Leucosinapis alba L.) seeds test, which evaluate length of the primary root after three days of germination. Another method was measurement of the heavy metal concentration in the dry tissue using mineralisation and atomic absorption spectroscopy. Metal concentration in the dry tissue was measured also in sedges cultivated in vitro in little boxes for 1 and 2 weeks. CuO nanoparticles (100 mg/l) were examined in detail using False Fox-sedge (Carex Otrubae Podp.). Evaluated markers were: the content of photosynthetic pigments (spectrophotometric), the content of proline (reaction with ninhydrin), peroxidase activity (using guajacol), catalase activity (using hydrogen...
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