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Testování genetické čistoty hybridního osiva zelí hlávkového pomocí molekulárních metod
 O'Shea, Denisa
This diploma thesis deals with testing of hybrid seeds genetic purity in three varieties of head cabbage (Brassica oleracea var. capitata L.). The suitability of SSR primers chosen according to an earlier screening carried out as a part of a research project in the workplace of Mendeleum and hybrid seeds genetic purity in head cabbage by SSR molecular method was tested. Among the total of 8 SSR primers screened, only 5 could produce both parent specific markers and therefore they were able to differentiate hybrid offspring and its corresponding parental lines, by which crossbreeding they originated. For each variety, 70 samples of hybrid offspring and 2 samples of parental lines were tested always in two sets. By using these primers, the genetic purity of the variety D - F1 was determinated as 100% and of the varieties H - F1 and T - F1 as 98.57%. The results of this thesis have the potential to be used in practice by a commercial company that has provided the seed samples for testing.
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