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Analytical evaluation of selected drugs using HPLC
Mingas, Panagiotis - Dimitrios ; Kovaříková, Petra (advisor) ; Kučera, Radim (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Kralove Department of Pharmaceutical Chemistry and Drug Control Candidate: Panagiotis Mingas Supervisor: Assoc. Prof., PharmDr. Petra Kovaříková, Ph.D. Title of diploma thesis: Analytical evaluation of selected drugs using HPLC In the developing pharmaceutical industry, HPLC is one of the most frequently used separation methods. Beyond drug discovery and development, HPLC has many other applications in various fields of pharmaceutical analysis. Aroylhydrazone iron chelators are potential drug candidates which both in vitro and in vivo pharmacodynamics studies demonstrated promising antioxidant and cardioprotective properties. Salicylaldehyde isonicotinoyl hydrazone (SIH) is the compound of the aroylhydrazone class that was the most thoroughly studied. The results that were obtained showed that its hydrazone bond makes SIH susceptible to hydrolysis in biological materials, decreasing its biological half- life. Various novel derivatives were developed in the direction of improving the stability of SIH in biological materials. The main aim of the research was firstly, to develop suitable chromatographic methods for the analysis of 8 novel iron chelators of the aroylhydrazone class (H21, H22, H23, H24, H25, H26, H32, H54) and secondly, utilize...
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