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Biological activity of plant metabolites XXIV. Influence of alkaloids from some species of the genus Fritillaria L. on activity of acetylcholinesterase and butyrylcholinesterase.
Mervartová, Markéta ; Opletal, Lubomír (advisor) ; Chlebek, Jakub (referee)
Mervartová, M.: Biological activity of plant metabolites XXIV. Influence of alkaloids from some species of the genus Fritillaria L. on activity of acetylcholinesterase and butyrylcholinesterase. Diploma thesis, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical botany and Ecology, Hradec Králové 2013, 60 s. Biological activity of extracts to human acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) was carried out. Extracts were prepared from ten chosen species of the genus Fritillaria which could be perspective for the treatment of Alzheimer's disease: F. camtschatcensis (bulb), F. eduardii (bulb), F. elwesii (bulb), F. graeca (bulb), F. hermonis var. amana (bulb), F. imperialis (herb), F. meleagris var. alba (bulb), F. michailovskyi (bulb), F. minuta (bulb), F. ussuriensis (bulb). Sumary (ethanol) and alkaloidal (ethylacetate) extract were prepared from each sample. The sumary extract was obtained by extraction of pulverized drug by 95% ethanol. The alkaloidal extract was made by following dissolution in 2% H2SO4, filtration, alkalization by 10% Na2CO3 on pH approx. 10 and final extraction of alkaloidal bases into ethylacetate. The inhibitory activity of these extracts to human AChE (from red blood cells) and BuChE (from plasma) was...
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