|
|
Residual analysis of botanical pesticides using separation methods
Medová, Tereza ; Nový, Pavel (advisor) ; Táborský, Jan (referee)
Botanical pesticides that include essential oils are considered to be one of the possible alternatives to synthetic chemical pesticides in plant protection, therefore it is important to develop our knowledge about these natural substances. This Master thesis comprises the residual analysis of selected compounds contained in Cymbopogon citratus Stapf, Pelargonium graveolens L'Hér and Thymus vulgaris L. essential oils after their application on plant material. The chemical composition of each essential oil was initially analyzed by gas chromatography - mass spectrometry (GC - MS). This analysis helped to select three representative compounds for each essential oil and the residues were determined for each of them. Citral, Geranyl-acetate and Limonene were selected for C. citratus, b-Citronellol, Geraniol and Linalool for P. graveolens and Thymol, p-Cymene and g-Terpinene for T. vulgaris. Two new methods to determine botanical pesticide residues were developed. The new methods should provide faster and easier analysis. One of them uses Solid Phase Microextraction method (SPME). The other one, that was applied in the residual analysis, uses hexane for extraction. The results show that essential oils' persistence is very low on the surface of plant material, which would imply they are environmentally friendly and safe for human health assuming correct manipulation with these substances.
|
guest ::
