National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Interakce makrofágů a buněk rakovinné prostatické linie PC-3
Mazalová, Lenka
This dissertation on the theme Interaction of macrophages and the prostate cancer cell line PC-3 was focused on the initial mapping of interactions between human mac-rophages and the prostate cancer cell line PC-3. Microscopic and molecular-genetic methods have been used for analysis. The structure and ultrastructure of macrophages and PC-3 cells have been de-scribed. The description of cell morphology in interaction with each other has been de-voted to processes associated with adherence of macrophages on PC-3, eferocytosis and phagocytosis. Cultivation of PC-3 with plumbagine showed an increase of apoptotic cells. Cultivation of macrophages with PC-3 supernatant has shown that solubiling fac-tors in supernatant may have an effect on inducing cell death in macrophages. In our study, the supernatant did not affect the production of TNF alfa, IL-12, IL-10 or IL-6 by macrophages. A time lapse video was created to show physical interactions of macro-phages and invasive cell. All obtained results show that there are interactions between the cancer cells and immune system cells that have not yet been discovered.

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