|
|
Diurnal changes of Rubisco content and its activity under ambient and elevated concentrations of carbon dioxide
Matulková, Zuzana ; Zemanová, Jana (referee) ; Hrstka, Miroslav (advisor)
In this diploma thesis, the diurnal changes of initial and total Rubisco activity and Rubisco enzyme content in beech (Fagus sylvatica) were studied under conditions of ambient (A) CO2 concentration (350 µmol.mol-1) and elevated (E) CO2 concentration (700 µmol.mol-1) during the day. Samples were taken on July 8th (from 10:00 to 21:30), on July 9th (from 04:00 to 12:00) and then on July 22th (from 04:00 to 21:30). The initial and total activity were measured spectrophotometrically and the activation level was calculated from the ratio of initial and total activities. Rubisco enzyme content was determined by SDS-PAGE method and the initial and total specific activity were calculated from the ratio of initial or total activity and Rubisco enzyme content. In our experiment no statistically significant difference was found between Rubisco activities in beeches cultivated under conditions of ambient CO2 concentration and elevated CO2 concentration, so any down-regulation of Rubisco activity did not appear under the influence of ambient CO2 concentration. Diurnal changes of Rubisco activities showed only statistically nonsignificant fluctuation. After daylight no significant increase of total Rubisco activity was observed, which demonstrates the absence or the immaterial effect of CA1P night inhibitor in beech. Intensive fluctuation of the activation level (40–90 %) proves the regulation of photosynthesis during the day via Rubisco enzyme carbamylation. Rubisco enzyme content in beech cultivated under conditions of ambient CO2 concentration was lower than that one in beech cultivated under conditions of elevated CO2 concentration. Then down-regulation of Rubisco enzyme content is presented under conditions of ambient CO2 concentration. Before daylight Rubisco enzyme content showed an indistinctive minimum on the level of statistical significance, during the day only nonrelevant fluctuation was noticed.
|
|
|
Jenny Nimmo´s Charlie Bone as a Hero of the Contemporary English Children´s Fantasy
MATULKOVÁ, Zuzana
The bachelor thesis deals with Jenny Nimmo´s contemporary English children´s fantasy. The first part is a brief introduction to the Nimmo´s life, because the writer is not a well known artist in our country. The next part describes a progression and signs of fantasy. Fantasy is connected with Gothic literature and Nimmo is typical of her usage of symbols of Welsh legends. The thesis also deals with an issue of secret, which is typical for several figures. The next part is an analysis of particular stories, figures, places, rituals and punishments. The final part compares the main character with Harry Potter, written by J. K. Rowling.
|
|
|
Diurnal changes of Rubisco content and its activity under ambient and elevated concentrations of carbon dioxide
Matulková, Zuzana ; Zemanová, Jana (referee) ; Hrstka, Miroslav (advisor)
In this diploma thesis, the diurnal changes of initial and total Rubisco activity and Rubisco enzyme content in beech (Fagus sylvatica) were studied under conditions of ambient (A) CO2 concentration (350 µmol.mol-1) and elevated (E) CO2 concentration (700 µmol.mol-1) during the day. Samples were taken on July 8th (from 10:00 to 21:30), on July 9th (from 04:00 to 12:00) and then on July 22th (from 04:00 to 21:30). The initial and total activity were measured spectrophotometrically and the activation level was calculated from the ratio of initial and total activities. Rubisco enzyme content was determined by SDS-PAGE method and the initial and total specific activity were calculated from the ratio of initial or total activity and Rubisco enzyme content. In our experiment no statistically significant difference was found between Rubisco activities in beeches cultivated under conditions of ambient CO2 concentration and elevated CO2 concentration, so any down-regulation of Rubisco activity did not appear under the influence of ambient CO2 concentration. Diurnal changes of Rubisco activities showed only statistically nonsignificant fluctuation. After daylight no significant increase of total Rubisco activity was observed, which demonstrates the absence or the immaterial effect of CA1P night inhibitor in beech. Intensive fluctuation of the activation level (40–90 %) proves the regulation of photosynthesis during the day via Rubisco enzyme carbamylation. Rubisco enzyme content in beech cultivated under conditions of ambient CO2 concentration was lower than that one in beech cultivated under conditions of elevated CO2 concentration. Then down-regulation of Rubisco enzyme content is presented under conditions of ambient CO2 concentration. Before daylight Rubisco enzyme content showed an indistinctive minimum on the level of statistical significance, during the day only nonrelevant fluctuation was noticed.
|
|
|
Influence of leaf age on the Rubisco content
Matulková, Zuzana ; Zemanová, Jana (referee) ; Hrstka, Miroslav (advisor)
The bachelor’s thesis describes the influence of leaf senescence on the Rubisco enzyme content in Fagus sylvatica, grown both in normal and elevated athmospheric carbon dioxide concentration. Tha samples were collected at the Station of experimental ecology, Institute of systems biology and ecology, Academy of Sciences of the Czech Republic, White Cross in Moravian-Silesian Beskydy. To specify the determination three series of samples were taken in following order - the first one on 22nd May 2007, the second one on 24th July 2007, and the third one then on 4th September 2007.The Rubisco enzyme content in leafs was measured by the SDS-PAGE method. In Fagus species grown in elevated carbon dioxide concentration we observed the depression of Rubisco enzyme content during the vegetation period. Reversely in Fagus species grown in normal carbon dioxide concentration the Rubisco enzyme content was increasing.
|
guest ::
RSS feed.