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Development of buildable areas in the zoning plans of the South Bohemian Region
MORAVCOVÁ, Vendula
Ever since mankind left the nomadic way of life and began to build permanent settlements from settlements to large cities, the development of settlements has its own order and structure. Although the rules for the development of municipalities are rooted in legislation, they are often neglected and the municipality's interest in expanding into free areas is prioritized over the interests of existing residents, the environment, and local farmers. The goal of this work was, based on the analysis of spatial planning documentation and other freely available materials, to obtain knowledge about the driving forces that influence the delimitation of buildable areas and their functional classification. A set of fourteen municipalities within the South Bohemian Region was chosen for this work. Based on statistical analyses carried out in the CANOCO program, it was found that the most important factor for the development of the territory is its current formation, but above all the level of technical infrastructure and civic amenities.
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Study of genetic diversity of Pectinatella magnifica (Leidy, 1851) colonies
MORAVCOVÁ, Vendula
Pectinatella magnifica is a freshwater organism from the bryozoan group, living in oligotrophic and mesotrophic waters where the temperature reaches 20°C during the year. It was first found and described by Joseph Leidy in the Philadelphia area in 1851. Although the original place of occurrence is North America, it is currently known as an invasive organism on other continents as well. Representatives of the species Pectinatella magnifica live in colonies with a characteristic spherical shape, which makes it easy to recognize and identify. These spherical colonies are covered with a community of zooids that produce a gel-like substance filling the colony itself. The reproduction of bryozoans is both sexual and asexual, with the non-sexual part exceeding the sexual part. The product of asexual reproduction are internal buds, so-called statoblasts, equipped with hooks, thanks to which they can attach to any substrate, which enables easy distribution even over long distances through otherwise difficult terrain. Although this is an ancient group with a predominance of asexual, therefore clonal, reproduction, the sexual part of reproduction enables the emergence of mutations and adaptation to new conditions. The dissertation is focused on determining the genetic diversity of colonies taken from the territory southern Bohemia and the surrounding area using molecular marker techniques. Two molecular techniques were used for processing genetic analyses, namely AFLP (Amplified Fragment Length Polymorphism) and ISSR (Inter Simple Sequence Repeat). Both techniques use universal primers to determine similarity between organisms.
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Study of genetic diversity of colonies Pectinatella magnifica
MORAVCOVÁ, Vendula
Pectinatella magnifica is a colony of freshwater organisms, which are most often found on the submerged parts/branches of coastal plants, but also fixed to stones, growing at the bottom of ponds, or free - floating. These organisms can be found mostly in oligotrophic to mesotrophic waters, and appear to thrive in temperatures that reach 20°C during colonisation. They were first found and described in Philadelphia in 1851, by Joseph Leidy. Although Pectinatella magnifica originates from North America, it has now become an invasive species on the other continents. The first recorded occurrence in Europe was during the 1980s in Hamburg, where it was probably transported on ships' hulls. In the Czech Republic it was recorded for the first time in rivers Vltava and Labe in the first half of the 20 th century. The most common method of its propagation is through internal buds, called statoblasts, fitted with hooks that make it able to cling to any surface, and allow easy distribution over long distances and otherwise impossible terrain. This study uses AFLP analysis of statoblast DNA to analyse the genetic diversity within P. magnifica colonies themselves, changes over time, and between different sites within the Protected Landscape Area (PLA) and a Biosphere Reserve (BR) of Třeboňsko, namely ponds Veseli I, Staňkovský, Nový Kanclíř, Cep, Vlkov and Nový Lipnický.
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Analysis of metabolome effects of hop (H. lupulus) transcription factors in heterologous of Petunia hybrida
MORAVCOVÁ, Vendula
Plant transformation is now a key research in plant biology, and also is a unique practical tool in the improvement of varieties of cultivated plants. The aim of this work was to obtain transgenic plants Petunia x hybrida containing transgene nptII (kanamycin resistance gene) using the indirect transformation by Agrobacterium tumefaciens. In terms of in-vivo was grown from seeds experimental material in the form of plant Petunia x hybrida cv. Andrea. For the experiments three different constructs HLWD40 3278-80 and HLbHLH 3577 and HLbHLH 3677 GFP were used. The leaf explants from Petunia plants were prepared. Explants were transferred using tweezers into the crucible containing liquid ? MS and prepared bacteria were added. Thus prepared explants remained in bacteria the next day, when they were transferred to the regenerating solid ground. The explants formed callus gradually (clusters of cells) which regenerate a new plant. Transformation should satisfy the condition that the transformed plants should be fertile. For this reason, an attempt was made crossing the transformed plants were crossed Pap1 1527/2 and Pap1 1572/4 with plants crossed HLWD 40 3278-80. The grown plants were analysed by a PCR reaction. The explants began during the first 2-4 weeks after transfromation to form callus and during the next week to remove the first regenerants. At one exulant fell averaged around 7 regenerants and the explants regenerated by construct HLbHLH 3577 regenerate better than regenerants transformed with construct HLWD 40 3278-80. During the transformation we obtained regenerants. With "tissue" PCR, it was found that 15 plants carried the gene for kanamycin resistance. Transformation efficiency was 3.75%.
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