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Influence of antiparasitic baths of fish on haematological and biochemical indicators
MECKO, Jozef
The aim of the diploma thesis was to evaluate the influence of antiparasitic baths on selected biochemical and haematological parameters in the blood samples of fish. According to the results the application schema will be specified or amended such alterations will be substantiated. A test was performed on the fry of carp (naked and scaly form) to evaluate the influence of the peractetic acid with the help of biochemical and haematological parameters and with the histopathological examination. The common carp was exposed to the concetration 1 mg?l-1 and 2 mg?l-1 of PAA. Three days after application was taken the blood samples from fish and the biochemical and haematological parameters were determined. Than was taken a sample of skin with the musculature from fish to be sent for the histopathological examination in University of Veterinary and Pharmaceutical Sciences in Brno. Based on the results the naked form of the carp has been declared to be more sensitive to the application of PAA. All the observed changes in haematological and biochemical parameters were within physiological range, so it can be stated, that the application schema of PAA has not a signifiant negative influence on the health of the tested fish.
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Influence of water temperature on toxicity of free ammonia for fish and on amount of ammonia produced by fish
MECKO, Jozef
Determination of acute toxicity of free ammonia for fish in dependence on water temperature and determination of production of ammonia by fish in dependence of water temperature It was made a test of acute toxicity on akutní toxicity on Poecilia reticulata according to ČSN EN ISO 7346-2 and determination of acute lethal toxicity of substances for fish. Next was made test on excretion of ammonia by common carp in different temperatures of water. Fish were divided after a two pieces into basins.The water temperature was kept in basins on two level. Next test was realized in a similar way with a difference, that an amyloid gel with an addition of NH4Cl was aplicated by a probe to the digestive tract of fish. To control fish were aplicated the amyloid gel without an addition of NH4Cl. It was observed concetration of ammonia in water and the fish were bleeded to set haematocrit and biochemical values. On grounds of these values will be analyzed production of ammonia by one species of fish in dependence on water temperature.
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