National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Physiological, structural, and biochemical leaf traits of selected Poaceae species involved in oxidative stress protection and acclimation to different light conditions
Hunt, Lena Macrie ; Albrechtová, Jana (advisor) ; Lux, Alexander (referee) ; Šantrůček, Jiří (referee)
This thesis examines physiological, structural, and biochemical leaf traits in grasses (Poaceae family) and how they contribute to oxidative stress protection. Light is a major factor contributing both to oxidative stress in plants and the induction of protective mechanisms at the leaf level. The agriculturally important species barley (Hordeum vulgare) was used to investigate responses to varying irradiance and atmospheric CO2 levels. Barley was further used as a model organism to study the influence of different spectral qualities on oxidative protective mechanisms, particularly phenolic compound induction. This thesis also examines the protective leaf functional traits of wild grass species (Nardus stricta, Calamagrostis villosa, Molinia caerulea, and Deschampsia cespitosa) in a high-irradiance arctic-alpine tundra grassland. Phenolic compounds are important protective secondary metabolites in plants that protect against oxidative stress from high irradiance. This thesis evaluates phenolic compounds and their contributions to plant stress tolerance and introduces a novel method of quantifying the histochemical detection of phenolics in leaf cross-sections. Key results indicate that light quantity and quality play major influential roles in the accumulation of phenolic compounds. High irradiance...
Organization of the microtubular cytoskeleton during the formation of Casparian band in root endodermal cells of Allium cepa
Macháčová, Kateřina ; Soukup, Aleš (advisor) ; Lux, Alexander (referee)
103 7 Summary Organization of the Microtubule Cytoskeleton during the Formation of a Casparian Band in Root Endodermal Cells of Allium cepa This thesis is focused on the process of Casparian bands initiation in radial and transverse cell walls of the root endodermis of Allium cepa. The Casparian band is characterized by a chemical modification of the cell wall in the position of the Casparian band followed by the formation of the tight binding of a plasma membrane to the cell wall. We assume that cortical microtubules can play an important role in both processes searching for changes of cortical array during the development of proendodermis and during the foundation of the Casparian band in the endodermis. The classical method of the microtubules visualisation in roots was modified by the application of microwaves during the process of fixation. The tissues were observed with the fluorescence and confocal microscopy. Image postprocessing and construction of 3D-models were subsequently used. Proendodermal cells in Allium cepa are recognized for the first time among other root cells in the distance of about 2 mm from the root tip. The Casparian band is firstly detected in the distance of about 8 mm from the root apex by means of autofluorescence of the Casparian band. The cell wall of the Casparian band...

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