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Application of Selected Methods for Oxidative Stress Analysis
Lízalová, Martina ; Tremlová, Bohuslava (referee) ; Beklová, Miroslava (referee) ; Vávrová, Milada (referee) ; Lojek, Antonín (advisor)
Chronic pancreatitis (CP) is a heterogeneous disease defined as chronic inflammatory changes of the pancreatic tissue caused by variety of aetiologies. Oxidative stress accompanying the inflammatory processes has been suggested as an important factor contributing to CP development. The aim of this study was to determine levels of lipid peroxidation products malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), together with nitrites, the total antioxidant capacity, cytokines, biochemical and haematological parameters in the plasma of patients with CP and control subjects. Levels of MDA and 4-HNE were analyzed using high-performance liquid chromatography. The total antioxidant capacity of plasma against peroxyl radicals was evaluated using chemiluminescence determination. Nitrites were determined using Griess reaction. Cytokines - TNF-alfa; TNF RI; PDGF-AB; TGF-beta, together with myeloperoxidase and hyaluronan were determined using ELISA Kits. Biochemical and haematological parameters were measured by standard methods.
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Czech asylum law between 16th and 18th century
Lojek, Antonín ; Adamová, Karolina (advisor) ; Soukup, Ladislav (referee) ; Hrdina, Antonín (referee)
This thesis deals mainly with Czech asylum law from early 16th to late 18th centuries. The history of asylum is very important for understanding contemporary asylum issues, however the focus of asylum historians, intense as it is, is mainly aimed at recent asylum history - in the 19th , 20th and 21st centuries. The aim of the author of this thesis was to show the history of asylum in preceding times, before the Vienna Congress and the 1848 revolutions, to point out the various forms of asylum law in this era and to try to answer the question of continuity of development of asylum law in its historical context. The main focus of the thesis is asylum law of the above period in Czech lands, its legislative bases in the forms of statutes and treaties and its implementation in practice as shown by historical sources. However, as the Czech lands of 16th to 18th centuries were not an isolated island, but rather one of the focal points of wider European society heavily linked to the surrounding developments, this thesis also deals with this wider context of the phenomenon studied. Asylum Law of the 16th to 18th centuries was diverse and open, but also ambivalent, haunted by difficult problems and conflicts. However, its main importance lay primarily in the fact that it dampened or restricted the severe...
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Application of Selected Methods for Oxidative Stress Analysis
Lízalová, Martina ; Tremlová, Bohuslava (referee) ; Beklová, Miroslava (referee) ; Vávrová, Milada (referee) ; Lojek, Antonín (advisor)
Chronic pancreatitis (CP) is a heterogeneous disease defined as chronic inflammatory changes of the pancreatic tissue caused by variety of aetiologies. Oxidative stress accompanying the inflammatory processes has been suggested as an important factor contributing to CP development. The aim of this study was to determine levels of lipid peroxidation products malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), together with nitrites, the total antioxidant capacity, cytokines, biochemical and haematological parameters in the plasma of patients with CP and control subjects. Levels of MDA and 4-HNE were analyzed using high-performance liquid chromatography. The total antioxidant capacity of plasma against peroxyl radicals was evaluated using chemiluminescence determination. Nitrites were determined using Griess reaction. Cytokines - TNF-alfa; TNF RI; PDGF-AB; TGF-beta, together with myeloperoxidase and hyaluronan were determined using ELISA Kits. Biochemical and haematological parameters were measured by standard methods.
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Miter - more than Just Clothing in Canon and Secular Law
Lojek, Antonín
The author in his article points out the fact that miter is not just a garment or a piece of clothing but it was also understood as insignia, i.e. a sign of rank, representing the power of secular and ecclesiastical persons. Miter represented the legitimate right of certain persons to perform certain functions and also indicated their social status.
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