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Biological effect of plant metabolites II. Isolation of alkaloids from Eschscholzia californica Cham.
Linzerová, Michaela ; Karlíčková, Jana (advisor) ; Macáková, Kateřina (referee)
Linzerová, M.: A Biological Effect of Plant Metabolits II. The Isolation of Alkaloids from Eschscholzia californica Cham. A diploma work, Charles University in Praque, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové 2009, 66 pages Extract J1 from plant Eschscholzia californica Cham. was separated. The separation was performed on a chromatografic column filled by silica gel, mixture of chloroform-ethanol 100+0 to 45+55 was chosen as eluent. For detection was taken thin-layer chromatography. A polar mixture of ethanol-acetic acid-water (60+30+10) was chosen as eleunt mixture. Detection was evaluated by UV radiation and spray of Dragendorff reagent. The fractions containing the highest concentracion of alkaloids were coupled and purified. Two different alkaloids were obtained. The structures of these alkaloids were found out by NMR method. Keywords: Californian Poppy, Eschscholzia californica, alkaloids, column chromatography, Alzheimer disease, benzophenanthridin alkaloids
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Biological effect of plant metabolites II. Isolation of alkaloids from Eschscholzia californica Cham.
Linzerová, Michaela ; Karlíčková, Jana (advisor) ; Macáková, Kateřina (referee)
Linzerová, M.: A Biological Effect of Plant Metabolits II. The Isolation of Alkaloids from Eschscholzia californica Cham. A diploma work, Charles University in Praque, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové 2009, 66 pages Extract J1 from plant Eschscholzia californica Cham. was separated. The separation was performed on a chromatografic column filled by silica gel, mixture of chloroform-ethanol 100+0 to 45+55 was chosen as eluent. For detection was taken thin-layer chromatography. A polar mixture of ethanol-acetic acid-water (60+30+10) was chosen as eleunt mixture. Detection was evaluated by UV radiation and spray of Dragendorff reagent. The fractions containing the highest concentracion of alkaloids were coupled and purified. Two different alkaloids were obtained. The structures of these alkaloids were found out by NMR method. Keywords: Californian Poppy, Eschscholzia californica, alkaloids, column chromatography, Alzheimer disease, benzophenanthridin alkaloids
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